Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng; S. Y. Yang; Chin Yih Hong; C. M. Liu; P. S. Tsai; H. C. Yang; C. C. Wu

doi:10.1063/1.2207990

Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng^*, S. Y. Yang, Chin Yih Hong, C. M. Liu, P. S. Tsai, H. C. Yang, C. C. Wu

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

Original language	English
Article number	252506
Journal	Applied Physics Letters
Volume	88
Issue number	25
DOIs	https://doi.org/10.1063/1.2207990
Publication status	Published - 2006 Jun 19

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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title = "Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein",

abstract = "In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.",

author = "Horng, {H. E.} and Yang, {S. Y.} and Hong, {Chin Yih} and Liu, {C. M.} and Tsai, {P. S.} and Yang, {H. C.} and Wu, {C. C.}",

note = "Funding Information: This work is supported by the National Science Council of Taiwan under Grant Nos. 94-2120-M-003-002, 94-2112-M-003-018, 94-2112-M-003-004, and 94-2212-E-001, 95-2752-M-002-016-PAE.",

year = "2006",

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doi = "10.1063/1.2207990",

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T1 - Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

AU - Horng, H. E.

AU - Yang, S. Y.

AU - Hong, Chin Yih

AU - Liu, C. M.

AU - Tsai, P. S.

AU - Yang, H. C.

AU - Wu, C. C.

N1 - Funding Information: This work is supported by the National Science Council of Taiwan under Grant Nos. 94-2120-M-003-002, 94-2112-M-003-018, 94-2112-M-003-004, and 94-2212-E-001, 95-2752-M-002-016-PAE.

PY - 2006/6/19

Y1 - 2006/6/19

N2 - In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

AB - In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

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Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

Abstract

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