Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng, S. Y. Yang, Chin Yih Hong, C. M. Liu, P. S. Tsai, H. C. Yang, C. C. Wu

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)

    Abstract

    In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

    Original languageEnglish
    Article number252506
    JournalApplied Physics Letters
    Volume88
    Issue number25
    DOIs
    Publication statusPublished - 2006 Jun 19

    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

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