Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng, S. Y. Yang, Chin Yih Hong, C. M. Liu, P. S. Tsai, H. C. Yang, C. C. Wu

Research output: Contribution to journalArticle

48 Citations (Scopus)


In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

Original languageEnglish
Article number252506
JournalApplied Physics Letters
Issue number25
Publication statusPublished - 2006 Jun 19


ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this