Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein

H. E. Horng, S. Y. Yang, Chin Yih Hong, C. M. Liu, P. S. Tsai, H. C. Yang, C. C. Wu

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In this work, we developed immunomagnetic detection techniques for detecting human C-reactive protein (CRP). To enhance the technique's sensitivity to human CRP, biofunctionalized magnetic nanoparticles were used as markers, and a superconductive quantum interference device gradiometer system was adapted to measure the saturated magnetization of magnetically labeled CRP-anti-CRP immune complexes. Sensitivity for human CRP was found to be as high as 1 ng in 0.1 ml, i.e., 10 ng/ml in concentration. This sensitivity is much higher than that of conventional enzyme linked immunosorbent assay by one order of magnitude.

Original languageEnglish
Article number252506
JournalApplied Physics Letters
Volume88
Issue number25
DOIs
Publication statusPublished - 2006 Jun 19

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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