Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

C. Y. Wang; T. W. Yang; D. Shen; K. L. Chen; J. M. Chen; S. H. Liao; J. J. Chieh; H. C. Yang; L. M. Wang

doi:10.1063/1.4979142

Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

C. Y. Wang, T. W. Yang, D. Shen, K. L. Chen^*, J. M. Chen, S. H. Liao, J. J. Chieh, H. C. Yang, L. M. Wang

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (T_B). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe₃O₄ were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of T_B increased with the concentration of the associated AFP. Furthermore, the dependence of T_B of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing T_B with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and Néel motions of magnetic moments in the BMNs.

Original language	English
Article number	133701
Journal	Applied Physics Letters
Volume	110
Issue number	13
DOIs	https://doi.org/10.1063/1.4979142
Publication status	Published - 2017 Mar 27

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.4979142

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@article{cc23b3686caf42f4b25d2f43b6203b9a,

title = "Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets",

abstract = "This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (TB). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe3O4 were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of TB increased with the concentration of the associated AFP. Furthermore, the dependence of TB of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing TB with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and N{\'e}el motions of magnetic moments in the BMNs.",

author = "Wang, {C. Y.} and Yang, {T. W.} and D. Shen and Chen, {K. L.} and Chen, {J. M.} and Liao, {S. H.} and Chieh, {J. J.} and Yang, {H. C.} and Wang, {L. M.}",

note = "Publisher Copyright: {\textcopyright} 2017 Author(s).",

year = "2017",

month = mar,

day = "27",

doi = "10.1063/1.4979142",

language = "English",

volume = "110",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Publising LLC",

number = "13",

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TY - JOUR

T1 - Bioassay using blocking temperature

T2 - Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

AU - Wang, C. Y.

AU - Yang, T. W.

AU - Shen, D.

AU - Chen, K. L.

AU - Chen, J. M.

AU - Liao, S. H.

AU - Chieh, J. J.

AU - Yang, H. C.

AU - Wang, L. M.

PY - 2017/3/27

Y1 - 2017/3/27

N2 - This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (TB). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe3O4 were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of TB increased with the concentration of the associated AFP. Furthermore, the dependence of TB of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing TB with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and Néel motions of magnetic moments in the BMNs.

AB - This paper reports a bioassay of alpha-fetoprotein (AFP) concentration achieved via the measurement of blocking temperature (TB). Biofunctionalized magnetic nanoparticles (BMNs) consisting of anti-alpha-fetoprotein coated onto dextran-coated magnetic nanoparticles composed of Fe3O4 were prepared and then conjugated with AFP biotargets. It was found that both the saturation magnetization and value of TB increased with the concentration of the associated AFP. Furthermore, the dependence of TB of the samples on magnetic field agreed with the interparticle interaction model. Thus, this study demonstrated a platform to detect biomarkers by characterizing TB with a sensitivity limit of 20 ppb of AFP. The promising results obtained for this bioassay can be attributed to the interparticle interactions and Néel motions of magnetic moments in the BMNs.

UR - http://www.scopus.com/inward/record.url?scp=85016461489&partnerID=8YFLogxK

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U2 - 10.1063/1.4979142

DO - 10.1063/1.4979142

M3 - Article

AN - SCOPUS:85016461489

SN - 0003-6951

VL - 110

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 13

M1 - 133701

ER -

Bioassay using blocking temperature: Interparticle interactions between biofunctionalized magnetic nanoparticles conjugated with biotargets

Abstract

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