Automatic calibration of the spatial position and orientation for the tomographic digital holographic microscopy

Li Chien Lin, Chau Jern Cheng, Yu Chun Hu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In previous study, a tomographic imaging system is built up for measuring the three-dimensional refractive index distribution inside the micrometer-sized biological cell by optically driven full-angle rotation scheme based on digital holographic microscopy, named as optical-driven tomographic DHM (OT-DHM) system. However, a small perturbation of the system will lead the inaccurate of the positions and the orientation of the micrometer-sized sample, thus the automatic calibration of the reconstructed phase images in the OT-DHM system is required. For this purpose, a novel model-based algorithm is proposed, in which we employ a 3-D ellipse shape for modeling the samples. The parameters of the ellipse-like shape on a small number of the projections are estimated and used them to build up the 3-D ellipse model of the samples. In advance, the reconstructed phase images are highly contaminated by the uneven background and coherence speckle noise. The block-based between-class criterion is used to suppress the effect of the non-uniform background, and the anisotropic diffusion process is utilized for the noise cleaning, including shot noise and speckles noise on the reconstructed phase. The boundary of the cell in each projection can be considered as the 2-D ellipse, and used to estimate the parameters of the 2-D ellipse. The established 3-D ellipse shape is applied for the calibration of the spatial positions and the orientations of the all other rotational angles. With the automatic calibration algorithm, the OT-DHM system can effectively reconstructed the three-dimensional refractive index distribution inside the micrometer-sized samples.

Original languageEnglish
Title of host publicationOptical Methods for Inspection, Characterization, and Imaging of Biomaterials IV
EditorsPietro Ferraro, Simonetta Grilli, Monika Ritsch-Marte, Monika Ritsch-Marte, Christoph K. Hitzenberger
PublisherSPIE
ISBN (Electronic)9781510627994
DOIs
Publication statusPublished - 2019 Jan 1
Externally publishedYes
EventOptical Methods for Inspection, Characterization, and Imaging of Biomaterials IV 2019 - Munich, Germany
Duration: 2019 Jun 242019 Jun 26

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11060
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceOptical Methods for Inspection, Characterization, and Imaging of Biomaterials IV 2019
CountryGermany
CityMunich
Period19/6/2419/6/26

Keywords

  • Anisotropic diffusion
  • Automatic calibration of the spatial position and orientation
  • Biological cell
  • Digital holographic microscopy
  • Model-based algorithm
  • Optical tweeze
  • Tomographic imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Automatic calibration of the spatial position and orientation for the tomographic digital holographic microscopy'. Together they form a unique fingerprint.

Cite this