Assessment of zinc finger orientations by residual dipolar coupling constants

Vickie Tsui, Leiming Zhu, Tai Huang Huang, Peter E. Wright, David A. Case

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Residual dipolar coupling constants measured in anisotropic solution contain information on orientations between internuclear vectors and the magnetic field, providing long-range information that may help determine the relative orientations of distinct domains in biomolecules. Here we describe the measurement and use of residual dipolar coupling restraints in the refinement of the structure of the complex of DNA with three zinc fingers of transcription factor IIIA (TFIIIA), measured in a DMPC/DHPC bicelle solution. These dipolar restraints were applied on a variety of orientations of the zinc finger domains (derived from crystallography, previous NMR studies, and systematic modeling) in order to examine the validity and sensitivity of using residual dipolar splittings to study interdomain orientations. The spread in interdomain angles between zinc fingers is reduced from 24°to 9°upon incorporation of dipolar restraints. However, the results also show that the ability to determine relative orientations is strongly dependent on the structural accuracy of the local domain structures.

Original languageEnglish
Pages (from-to)9-21
Number of pages13
JournalJournal of Biomolecular NMR
Volume16
Issue number1
DOIs
Publication statusPublished - 2000 Mar 7

Keywords

  • Dipolar couplings
  • Domain orientation
  • Transcription factor III A
  • Zinc fingers

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

Fingerprint Dive into the research topics of 'Assessment of zinc finger orientations by residual dipolar coupling constants'. Together they form a unique fingerprint.

  • Cite this