Assembly of the Caulobacter cell division machine

Erin D. Goley, Yi-Chun Yeh, Sun Hae Hong, Michael J. Fero, Eduardo Abeliuk, Harley H. Mcadams, Lucy Shapiro

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Cytokinesis in Gram-negative bacteria is mediated by a multiprotein machine (the divisome) that invaginates and remodels the inner membrane, peptidoglycan and outer membrane. Understanding the order of divisome assembly would inform models of the interactions among its components and their respective functions. We leveraged the ability to isolate synchronous populations of Caulobacter crescentus cells to investigate assembly of the divisome and place the arrival of each component into functional context. Additionally, we investigated the genetic dependence of localization among divisome proteins and the cell cycle regulation of their transcript and protein levels to gain insight into the control mechanisms underlying their assembly. Our results revealed a picture of divisome assembly with unprecedented temporal resolution. Specifically, we observed (i) initial establishment of the division site, (ii) recruitment of early FtsZ-binding proteins, (iii) arrival of proteins involved in peptidoglycan remodelling, (iv) arrival of FtsA, (v) assembly of core divisome components, (vi) initiation of envelope invagination, (vii) recruitment of polar markers and cytoplasmic compartmentalization and (viii) cell separation. Our analysis revealed differences in divisome assembly among Caulobacter and other bacteria that establish a framework for identifying aspects of bacterial cytokinesis that are widely conserved from those that are more variable.

Original languageEnglish
Pages (from-to)1680-1698
Number of pages19
JournalMolecular Microbiology
Volume80
Issue number6
DOIs
Publication statusPublished - 2011 Jun 1

Fingerprint

Caulobacter
Cytokinesis
Peptidoglycan
Cell Division
Caulobacter crescentus
Cell Cycle Proteins
Membranes
Cell Separation
Gram-Negative Bacteria
Carrier Proteins
Proteins
Bacteria
Population

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Goley, E. D., Yeh, Y-C., Hong, S. H., Fero, M. J., Abeliuk, E., Mcadams, H. H., & Shapiro, L. (2011). Assembly of the Caulobacter cell division machine. Molecular Microbiology, 80(6), 1680-1698. https://doi.org/10.1111/j.1365-2958.2011.07677.x

Assembly of the Caulobacter cell division machine. / Goley, Erin D.; Yeh, Yi-Chun; Hong, Sun Hae; Fero, Michael J.; Abeliuk, Eduardo; Mcadams, Harley H.; Shapiro, Lucy.

In: Molecular Microbiology, Vol. 80, No. 6, 01.06.2011, p. 1680-1698.

Research output: Contribution to journalArticle

Goley, ED, Yeh, Y-C, Hong, SH, Fero, MJ, Abeliuk, E, Mcadams, HH & Shapiro, L 2011, 'Assembly of the Caulobacter cell division machine', Molecular Microbiology, vol. 80, no. 6, pp. 1680-1698. https://doi.org/10.1111/j.1365-2958.2011.07677.x
Goley ED, Yeh Y-C, Hong SH, Fero MJ, Abeliuk E, Mcadams HH et al. Assembly of the Caulobacter cell division machine. Molecular Microbiology. 2011 Jun 1;80(6):1680-1698. https://doi.org/10.1111/j.1365-2958.2011.07677.x
Goley, Erin D. ; Yeh, Yi-Chun ; Hong, Sun Hae ; Fero, Michael J. ; Abeliuk, Eduardo ; Mcadams, Harley H. ; Shapiro, Lucy. / Assembly of the Caulobacter cell division machine. In: Molecular Microbiology. 2011 ; Vol. 80, No. 6. pp. 1680-1698.
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