Assaying Carcinoembryonic Antigens by Normalized Saturation Magnetization

Kai Wen Huang, Jen Jie Chieh, Jin Cheng Shi, Ming Hsien Chiang

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2 Citations (Scopus)

Abstract

Biofunctionalized magnetic nanoparticles (BMNs) that provide unique advantages have been extensively used to develop immunoassay methods. However, these developed magnetic methods have been used only for specific immunoassays and not in studies of magnetic characteristics of materials. In this study, a common vibration sample magnetometer (VSM) was used for the measurement of the hysteresis loop for different carcinoembryonic antigens (CEA) concentrations (ΦCEA) based on the synthesized BMNs with anti-CEA coating. Additionally, magnetic parameters such as magnetization (M), remanent magnetization (MR), saturation magnetization (MS), and normalized parameters (ΔMR/MR and ΔMS/MS) were studied. Here, ΔMR and ΔMs were defined as the difference between any ΦCEA and zero ΦCEA. The parameters M, ΔMR, and ΔMS increased with ΦCEA, and ΔMS showed the largest increase. Magnetic clusters produced by the conjugation of the BMNs to CEAs showed a ΔMS greater than that of BMNs. Furthermore, the relationship between ΔMS/MS and ΦCEA could be described by a characteristic logistic function, which was appropriate for assaying the amount of CEAs. This analytic ΔMS/MS and the BMNs used in general magnetic immunoassays can be used for upgrading the functions of the VSM and for studying the magnetic characteristics of materials.

Original languageEnglish
Article number277
JournalNanoscale Research Letters
Volume10
Issue number1
DOIs
Publication statusPublished - 2015 Dec 23

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Keywords

  • Biofunctionalized magnetic nanoparticles
  • Carcinoembryonic antigen
  • Magnetic clusters
  • Magnetic immunoassays
  • Saturation magnetization

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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