Aspirin disrupts the crosstalk of angiogenic and inflammatory cytokines between 4t1 breast cancer cells and macrophages

Chia Chien Hsieh, Chih Hsuan Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The tumor microenvironment is rich in multiple cell types that influence tumor development. Macrophages infiltrate tumors, where they are the most abundant immune cell population and secrete a number of cytokines. Aspirin acts as a chemopreventive agent against cancer development. This study investigated whether aspirin regulates crosstalk between breast cancer cells and macrophages. To study these interactions in a tumor microenvironment, a conditioned media was employed using 4T1 breast cancer cells cultured in RAW 264.7 cell-conditioned medium (RAW-CM), and a cocultured model of both cells was used. When 4T1 cells were cultured in the RAW-CM, there were increases in cell viability and secretion of the cytokines VEGF, PAI-1, TNF-α, and IL-6. Treatment with aspirin inhibited 4T1 cell growth and migration and MCP-1, PAI-1, and IL-6 production. In the coculture of both cells, aspirin inhibited secretion of MCP-1, IL-6, and TGF-ǎ. Furthermore, aspirin significantly decreased the M2 macrophage marker CD206, but increased M1 marker CD11c expression. In summary, aspirin treatment inhibited the crosstalk of 4T1 and RAW 264.7 cells through regulation of angiogenic and inflammatory mediator production and influenced the M1/M2 macrophage subtype. This highlighted that aspirin suppresses the tumor favorable microenvironment and could be a promising agent against triple-negative breast cancer.

Original languageEnglish
Article number6380643
JournalMediators of Inflammation
Volume2018
DOIs
Publication statusPublished - 2018 Jan 1

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Aspirin
Macrophages
Breast Neoplasms
Cytokines
Tumor Microenvironment
Conditioned Culture Medium
Interleukin-6
Plasminogen Activator Inhibitor 1
Cultured Cells
Triple Negative Breast Neoplasms
Neoplasms
Coculture Techniques
Vascular Endothelial Growth Factor A
Cell Movement
Cell Survival
Growth
Population
RAW 264.7 Cells

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Aspirin disrupts the crosstalk of angiogenic and inflammatory cytokines between 4t1 breast cancer cells and macrophages. / Hsieh, Chia Chien; Wang, Chih Hsuan.

In: Mediators of Inflammation, Vol. 2018, 6380643, 01.01.2018.

Research output: Contribution to journalArticle

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