Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon

Yuan Hsuan Liu, Jin Wu Tsai, Jia Long Chen, Wan Shan Yang, Pei Ching Chang, Pei Lin Cheng, David L. Turner, Yuchio Yanagawa, Tsu Wei Wang, Jenn Yah Yu

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Abstract

During development, cortical interneurons generated from the ventral telencephalon migrate tangentially into the dorsal telencephalon. Although Achaete-scute family bHLH transcription factor 1 (Ascl1) plays important roles in the developing telencephalon, whether Ascl1 regulates tangential migration remains unclear. Here, we found that Ascl1 promoted tangential migration along the ventricular zone/subventricular zone (VZ/SVZ) and intermediate zone (IZ) of the dorsal telencephalon. Distal-less homeobox 2 (Dlx2) acted downstream of Ascl1 in promoting tangential migration along the VZ/SVZ but not IZ. We further identified Eph receptor B2 (Ephb2) as a direct target of Ascl1. Knockdown of EphB2 disrupted the separation of the VZ/SVZ and IZ migratory routes. Ephrin-A5, a ligand of EphB2, was sufficient to repel both Ascl1-expressing cells in vitro and tangentially migrating cortical interneurons in vivo. Together, our results demonstrate that Ascl1 induces expression of Dlx2 and Ephb2 to maintain distinct tangential migratory routes in the dorsal telencephalon.

Original languageEnglish
Article number42895
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 2017 Mar 9

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EphA1 Receptor
Telencephalon
Lateral Ventricles
Homeobox Genes
Interneurons
Ephrin-A5
Basic Helix-Loop-Helix Transcription Factors
Ligands

ASJC Scopus subject areas

  • General

Cite this

Liu, Y. H., Tsai, J. W., Chen, J. L., Yang, W. S., Chang, P. C., Cheng, P. L., ... Yu, J. Y. (2017). Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. Scientific Reports, 7, [42895]. https://doi.org/10.1038/srep42895

Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. / Liu, Yuan Hsuan; Tsai, Jin Wu; Chen, Jia Long; Yang, Wan Shan; Chang, Pei Ching; Cheng, Pei Lin; Turner, David L.; Yanagawa, Yuchio; Wang, Tsu Wei; Yu, Jenn Yah.

In: Scientific Reports, Vol. 7, 42895, 09.03.2017.

Research output: Contribution to journalArticle

Liu, YH, Tsai, JW, Chen, JL, Yang, WS, Chang, PC, Cheng, PL, Turner, DL, Yanagawa, Y, Wang, TW & Yu, JY 2017, 'Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon', Scientific Reports, vol. 7, 42895. https://doi.org/10.1038/srep42895
Liu, Yuan Hsuan ; Tsai, Jin Wu ; Chen, Jia Long ; Yang, Wan Shan ; Chang, Pei Ching ; Cheng, Pei Lin ; Turner, David L. ; Yanagawa, Yuchio ; Wang, Tsu Wei ; Yu, Jenn Yah. / Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. In: Scientific Reports. 2017 ; Vol. 7.
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