AS1411 aptamer-conjugated Gd2O3:Eu nanoparticles for target-specific computed tomography/magnetic resonance/fluorescence molecular imaging

Tsungrong Kuo, Weiyun Lai, Chenghung Li, Yanjhan Wun, Huancheng Chang, Jinnshiun Chen, Panchyr Yang, Chiachun Chen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Europium-doped gadolinium oxide (Gd2O3:Eu) nanoparticles have been synthesized, and then their surfaces have been conjugated with nucleolintargeted AS1411 aptamer to form functionalized target-specific Gd2O3:Eu nanoparticles (A-GdO:Eu nanoparticles). The A-GdO:Eu nanoparticles present strong fluorescence in the visible range, high magnetic susceptibility, X-ray attenuation and good biocompatibility. The A-GdO:Eu nanoparticles have been applied to test molecular expression of nucleolin highly expressed CL1-5 lung cancer cells under a confocal microscope. Fluorescence imaging clearly reveals that the nanoparticles can be applied as fluorescent tags for cancer-targeting molecular imaging. Furthermore, taking together their excellent T 1 contrast and strong computed tomography (CT) signal, the A-GdO:Eu nanoparticles demonstrate a great capability for use as a dual modality contrast agent for CT and magnetic resonance (MR) molecular imaging. Animal experiments also show that the A-GdO:Eu nanoparticles are able to contrast the tissues of BALB/c mice using CT modality. Moreover, the obvious red fluorescence of A-GdO:Eu nanoparticles can be visualized in a tumor by the naked eye. Overall, our results demonstrate that the A-GdO:Eu nanoparticles can not only serve as new medical contrast agents but also as intraoperative fluorescence imaging probes for guided surgery in the near future. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)658-669
Number of pages12
JournalNano Research
Volume7
Issue number5
DOIs
Publication statusPublished - 2014 May

Keywords

  • aptamer
  • contrast agent
  • molecular imaging
  • nanoparticles synthesis

ASJC Scopus subject areas

  • Materials Science(all)
  • Electrical and Electronic Engineering

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