Arabidopsis Root-Abundant Cytosolic Methionine Sulfoxide Reductase B Genes MsrB7 and MsrB8 are Involved in Tolerance to Oxidative Stress

Chia Wen Li, Shu Hong Lee, Pei Shan Chieh, Choun Sea Lin, Yu Chie Wang, Ming Tsair Chan

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Excess reactive oxygen species (ROS) accumulation under various environmental stresses can damage intracellular polysaccharides, DNA, lipids and proteins. Methionine sulfoxide reductase (MSR) participates in a protein repair system that is one of the defensive mechanisms that diminishes oxidative destruction. In Arabidopsis, cytosolic MsrB7 and MsrB8 are oxidative stress-inducible protein repair enzymes that are abundant in the root. Here methyl viologen (MV) treatment was demonstrated to increase greatly the accumulation of H2O2 in MsrB7-knockdown, MsrB8-knockdown and wild-type Arabidopsis, but not in transgenic plants overexpressing MsrB7 or MsrB8. The reduction in H2O2 level under MV treatment in these overexpressing plants coincided with increased activity of glutathione S-transferase (GST), a herbicide-detoxifying enzyme. MsrB7 and MsrB8 are suggested to play an important role in defense against oxidative stress. Transgenic plants overexpressing MsrB7 or MsrB8 were viable and survived after MV and H2O2 treatment. Ectopic expression of specific cytosolic MsrB genes may be useful for application in crop improvement.

Original languageEnglish
Pages (from-to)1707-1719
Number of pages13
JournalPlant and Cell Physiology
Volume53
Issue number10
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Methionine Sulfoxide Reductases
Paraquat
paraquat
Arabidopsis
methionine
Oxidative Stress
oxidative stress
Genetically Modified Plants
transgenic plants
Genes
genes
proteins
Herbicides
Enzymes
Heat-Shock Proteins
enzymes
Glutathione Transferase
glutathione transferase
Polysaccharides
reactive oxygen species

Keywords

  • Arabidopsis thaliana
  • Glutathione S-transferase (GST)
  • Methionine sulfoxide reductase B (MsrB)
  • Methyl viologen (MV)

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Arabidopsis Root-Abundant Cytosolic Methionine Sulfoxide Reductase B Genes MsrB7 and MsrB8 are Involved in Tolerance to Oxidative Stress. / Li, Chia Wen; Lee, Shu Hong; Chieh, Pei Shan; Lin, Choun Sea; Wang, Yu Chie; Chan, Ming Tsair.

In: Plant and Cell Physiology, Vol. 53, No. 10, 01.10.2012, p. 1707-1719.

Research output: Contribution to journalArticle

Li, Chia Wen ; Lee, Shu Hong ; Chieh, Pei Shan ; Lin, Choun Sea ; Wang, Yu Chie ; Chan, Ming Tsair. / Arabidopsis Root-Abundant Cytosolic Methionine Sulfoxide Reductase B Genes MsrB7 and MsrB8 are Involved in Tolerance to Oxidative Stress. In: Plant and Cell Physiology. 2012 ; Vol. 53, No. 10. pp. 1707-1719.
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