Abstract
The Hadamard transform-gas chromatography/mass spectrometry (HT-GC/MS) technique was successfully employed to detect acetone, a biomarker for diabetes mellitus (DM) prediction, in human breath. Samples of exhaled breath were collected from four DM patients (one type-I and three type-II) and eight volunteers (nondiabetic healthy subjects), respectively. The gas samples, without any pretreatment, were simultaneously injected into a GC column through a Hadamard-injector based on Hadamard codes. Under optimized conditions, when cyclic S-matrix orders of 255, 1023 and 2047 were used, the S/N ratios of the acetone signals were substantially improved by 8.0-, 16.0- and 22.6-fold, respectively; these improvements are in good agreement with theoretically calculated values. We found that the breath acetone concentration levels in the four DM patients and the eight volunteers ranged from 1 to 10 ppmv and 0.1 to 1 ppmv, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 386-390 |
| Number of pages | 5 |
| Journal | Talanta |
| Volume | 120 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
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Keywords
- Acetone
- Diabetes mellitus (DM)
- Gas chromatography/mass spectrometry
- Glycohemoglobin A1c (HbA1c)
- Hadamard transform
- Human breath
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Applications of Hadamard transform-gas chromatography/mass spectrometry to the detection of acetone in healthy human and diabetes mellitus patient breath. / Fan, Gang Ting; Yang, Chien Lin; Lin, Cheng-Huang; Chen, Chien Chung; Shih, Chung Hung.
In: Talanta, Vol. 120, 01.01.2014, p. 386-390.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Applications of Hadamard transform-gas chromatography/mass spectrometry to the detection of acetone in healthy human and diabetes mellitus patient breath
AU - Fan, Gang Ting
AU - Yang, Chien Lin
AU - Lin, Cheng-Huang
AU - Chen, Chien Chung
AU - Shih, Chung Hung
PY - 2014/1/1
Y1 - 2014/1/1
N2 - The Hadamard transform-gas chromatography/mass spectrometry (HT-GC/MS) technique was successfully employed to detect acetone, a biomarker for diabetes mellitus (DM) prediction, in human breath. Samples of exhaled breath were collected from four DM patients (one type-I and three type-II) and eight volunteers (nondiabetic healthy subjects), respectively. The gas samples, without any pretreatment, were simultaneously injected into a GC column through a Hadamard-injector based on Hadamard codes. Under optimized conditions, when cyclic S-matrix orders of 255, 1023 and 2047 were used, the S/N ratios of the acetone signals were substantially improved by 8.0-, 16.0- and 22.6-fold, respectively; these improvements are in good agreement with theoretically calculated values. We found that the breath acetone concentration levels in the four DM patients and the eight volunteers ranged from 1 to 10 ppmv and 0.1 to 1 ppmv, respectively.
AB - The Hadamard transform-gas chromatography/mass spectrometry (HT-GC/MS) technique was successfully employed to detect acetone, a biomarker for diabetes mellitus (DM) prediction, in human breath. Samples of exhaled breath were collected from four DM patients (one type-I and three type-II) and eight volunteers (nondiabetic healthy subjects), respectively. The gas samples, without any pretreatment, were simultaneously injected into a GC column through a Hadamard-injector based on Hadamard codes. Under optimized conditions, when cyclic S-matrix orders of 255, 1023 and 2047 were used, the S/N ratios of the acetone signals were substantially improved by 8.0-, 16.0- and 22.6-fold, respectively; these improvements are in good agreement with theoretically calculated values. We found that the breath acetone concentration levels in the four DM patients and the eight volunteers ranged from 1 to 10 ppmv and 0.1 to 1 ppmv, respectively.
KW - Acetone
KW - Diabetes mellitus (DM)
KW - Gas chromatography/mass spectrometry
KW - Glycohemoglobin A1c (HbA1c)
KW - Hadamard transform
KW - Human breath
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U2 - 10.1016/j.talanta.2013.12.025
DO - 10.1016/j.talanta.2013.12.025
M3 - Article
C2 - 24468386
AN - SCOPUS:84891669100
VL - 120
SP - 386
EP - 390
JO - Talanta
JF - Talanta
SN - 0039-9140
ER -