Applications of Hadamard transform-gas chromatography/mass spectrometry to the detection of acetone in healthy human and diabetes mellitus patient breath

Gang Ting Fan, Chien Lin Yang, Cheng Huang Lin, Chien Chung Chen, Chung Hung Shih

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

The Hadamard transform-gas chromatography/mass spectrometry (HT-GC/MS) technique was successfully employed to detect acetone, a biomarker for diabetes mellitus (DM) prediction, in human breath. Samples of exhaled breath were collected from four DM patients (one type-I and three type-II) and eight volunteers (nondiabetic healthy subjects), respectively. The gas samples, without any pretreatment, were simultaneously injected into a GC column through a Hadamard-injector based on Hadamard codes. Under optimized conditions, when cyclic S-matrix orders of 255, 1023 and 2047 were used, the S/N ratios of the acetone signals were substantially improved by 8.0-, 16.0- and 22.6-fold, respectively; these improvements are in good agreement with theoretically calculated values. We found that the breath acetone concentration levels in the four DM patients and the eight volunteers ranged from 1 to 10 ppmv and 0.1 to 1 ppmv, respectively.

Original languageEnglish
Pages (from-to)386-390
Number of pages5
JournalTalanta
Volume120
DOIs
Publication statusPublished - 2014 Mar

Keywords

  • Acetone
  • Diabetes mellitus (DM)
  • Gas chromatography/mass spectrometry
  • Glycohemoglobin A1c (HbA1c)
  • Hadamard transform
  • Human breath

ASJC Scopus subject areas

  • Analytical Chemistry

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