Application of the solvent extraction technique to investigation of the anti-inflammatory activity of adlay bran

Din Wen Huang; Chi Hao Wu; Chun Kuang Shih; Chia Yu Liu; Ping Hsiao Shih; Tzong Ming Shieh; Ching I. Lin; Wenchang Chiang; Shih Min Hsia

doi:10.1016/j.foodchem.2013.08.071

Application of the solvent extraction technique to investigation of the anti-inflammatory activity of adlay bran

Din Wen Huang, Chi Hao Wu, Chun Kuang Shih, Chia Yu Liu, Ping Hsiao Shih, Tzong Ming Shieh, Ching I. Lin, Wenchang Chiang, Shih Min Hsia^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The current study utilised a bioassay-directed chemical analysis scheme to screen the anti-inflammatory activity of fractions and compounds from adlay bran (AB). Liquid-liquid extraction couple with liquid chromatography-mass spectrometry (LC-MS) was applied to the isolation, analysis and identification of active components in AB samples. Ethanol extracts of AB (ABE) and ethyl acetate extracts AB (ABEa) were obtained and further partitioned with different solvents. The results showed that among all 16 kinds of fractions from ABE and ABEa, ABEa-Ea-B (80% Ea/n-hexane sub-fraction from ABE-Ea) had the most potent inhibitory effects on NO production, iNOS and COX-2 expressions, and proinflammatory IL-6 and TNF-α secretion in lipopolysaccharide-activated RAW264.7 cells system. Mechanistic data from luciferase reporter-gene assay revealed that the anti-inflammatory action of ABEa-Ea-B may be associated with inhibition of NF-kB transcriptional activity. Notably, tangeretin, nobiletin, and p-hydroxybenzoic acid were found to be the main active compounds for the anti-inflammatory properties in ABEa-Ea-B.

Original language	English
Pages (from-to)	445-453
Number of pages	9
Journal	Food Chemistry
Volume	145
DOIs	https://doi.org/10.1016/j.foodchem.2013.08.071
Publication status	Published - 2014
Externally published	Yes

Keywords

Adlay bran
Anti-inflammation
Lipopolysaccharide
RAW 264.7 macrophages

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2013.08.071

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@article{8cde4c6fa8de4d8dbd0c0e53fe584ed8,

title = "Application of the solvent extraction technique to investigation of the anti-inflammatory activity of adlay bran",

abstract = "The current study utilised a bioassay-directed chemical analysis scheme to screen the anti-inflammatory activity of fractions and compounds from adlay bran (AB). Liquid-liquid extraction couple with liquid chromatography-mass spectrometry (LC-MS) was applied to the isolation, analysis and identification of active components in AB samples. Ethanol extracts of AB (ABE) and ethyl acetate extracts AB (ABEa) were obtained and further partitioned with different solvents. The results showed that among all 16 kinds of fractions from ABE and ABEa, ABEa-Ea-B (80% Ea/n-hexane sub-fraction from ABE-Ea) had the most potent inhibitory effects on NO production, iNOS and COX-2 expressions, and proinflammatory IL-6 and TNF-α secretion in lipopolysaccharide-activated RAW264.7 cells system. Mechanistic data from luciferase reporter-gene assay revealed that the anti-inflammatory action of ABEa-Ea-B may be associated with inhibition of NF-kB transcriptional activity. Notably, tangeretin, nobiletin, and p-hydroxybenzoic acid were found to be the main active compounds for the anti-inflammatory properties in ABEa-Ea-B.",

keywords = "Adlay bran, Anti-inflammation, Lipopolysaccharide, RAW 264.7 macrophages",

author = "Huang, {Din Wen} and Wu, {Chi Hao} and Shih, {Chun Kuang} and Liu, {Chia Yu} and Shih, {Ping Hsiao} and Shieh, {Tzong Ming} and Lin, {Ching I.} and Wenchang Chiang and Hsia, {Shih Min}",

note = "Funding Information: This study was supported by the Grants (100-2313-B-424-002-, NSC101-2313-B-038-005-, NSC101-2629-038-002-, and NSC101-2313-B-038-006-MY2), from National Science Council and supported by the Grants (TMU101-AE1-B16 and TMU101-AE1-B17), from Taipei Medical University, Taipei, Taiwan, Republic of China. ",

year = "2014",

doi = "10.1016/j.foodchem.2013.08.071",

language = "English",

volume = "145",

pages = "445--453",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Application of the solvent extraction technique to investigation of the anti-inflammatory activity of adlay bran

AU - Huang, Din Wen

AU - Wu, Chi Hao

AU - Shih, Chun Kuang

AU - Liu, Chia Yu

AU - Shih, Ping Hsiao

AU - Shieh, Tzong Ming

AU - Lin, Ching I.

AU - Chiang, Wenchang

AU - Hsia, Shih Min

N1 - Funding Information: This study was supported by the Grants (100-2313-B-424-002-, NSC101-2313-B-038-005-, NSC101-2629-038-002-, and NSC101-2313-B-038-006-MY2), from National Science Council and supported by the Grants (TMU101-AE1-B16 and TMU101-AE1-B17), from Taipei Medical University, Taipei, Taiwan, Republic of China.

PY - 2014

Y1 - 2014

N2 - The current study utilised a bioassay-directed chemical analysis scheme to screen the anti-inflammatory activity of fractions and compounds from adlay bran (AB). Liquid-liquid extraction couple with liquid chromatography-mass spectrometry (LC-MS) was applied to the isolation, analysis and identification of active components in AB samples. Ethanol extracts of AB (ABE) and ethyl acetate extracts AB (ABEa) were obtained and further partitioned with different solvents. The results showed that among all 16 kinds of fractions from ABE and ABEa, ABEa-Ea-B (80% Ea/n-hexane sub-fraction from ABE-Ea) had the most potent inhibitory effects on NO production, iNOS and COX-2 expressions, and proinflammatory IL-6 and TNF-α secretion in lipopolysaccharide-activated RAW264.7 cells system. Mechanistic data from luciferase reporter-gene assay revealed that the anti-inflammatory action of ABEa-Ea-B may be associated with inhibition of NF-kB transcriptional activity. Notably, tangeretin, nobiletin, and p-hydroxybenzoic acid were found to be the main active compounds for the anti-inflammatory properties in ABEa-Ea-B.

AB - The current study utilised a bioassay-directed chemical analysis scheme to screen the anti-inflammatory activity of fractions and compounds from adlay bran (AB). Liquid-liquid extraction couple with liquid chromatography-mass spectrometry (LC-MS) was applied to the isolation, analysis and identification of active components in AB samples. Ethanol extracts of AB (ABE) and ethyl acetate extracts AB (ABEa) were obtained and further partitioned with different solvents. The results showed that among all 16 kinds of fractions from ABE and ABEa, ABEa-Ea-B (80% Ea/n-hexane sub-fraction from ABE-Ea) had the most potent inhibitory effects on NO production, iNOS and COX-2 expressions, and proinflammatory IL-6 and TNF-α secretion in lipopolysaccharide-activated RAW264.7 cells system. Mechanistic data from luciferase reporter-gene assay revealed that the anti-inflammatory action of ABEa-Ea-B may be associated with inhibition of NF-kB transcriptional activity. Notably, tangeretin, nobiletin, and p-hydroxybenzoic acid were found to be the main active compounds for the anti-inflammatory properties in ABEa-Ea-B.

KW - Adlay bran

KW - Anti-inflammation

KW - Lipopolysaccharide

KW - RAW 264.7 macrophages

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U2 - 10.1016/j.foodchem.2013.08.071

DO - 10.1016/j.foodchem.2013.08.071

M3 - Article

C2 - 24128500

AN - SCOPUS:84883694042

SN - 0308-8146

VL - 145

SP - 445

EP - 453

JO - Food Chemistry

JF - Food Chemistry

ER -

Application of the solvent extraction technique to investigation of the anti-inflammatory activity of adlay bran

Abstract

Keywords

ASJC Scopus subject areas

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