Application of capillary electrophoresis-fluorescence line-narrowing spectroscopy for on-line spectral characterization of closely related analytes

D. Zamzow; C. H. Lin; G. J. Small; R. Jankowiak

doi:10.1016/S0021-9673(97)00499-8

Application of capillary electrophoresis-fluorescence line-narrowing spectroscopy for on-line spectral characterization of closely related analytes

D. Zamzow
, C. H. Lin
, G. J. Small
, R. Jankowiak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.

Original language	English
Pages (from-to)	73-80
Number of pages	8
Journal	Journal of Chromatography A
Volume	781
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(97)00499-8
Publication status	Published - 1997 Sept 26
Externally published	Yes

Keywords

DNA
Detection, electrophoresis
Fluorescence line-narrowing spectroscopy
Polynuclear aromatic hydrocarbons

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(97)00499-8

Cite this

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title = "Application of capillary electrophoresis-fluorescence line-narrowing spectroscopy for on-line spectral characterization of closely related analytes",

abstract = "Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.",

keywords = "DNA, Detection, electrophoresis, Fluorescence line-narrowing spectroscopy, Polynuclear aromatic hydrocarbons",

author = "D. Zamzow and Lin, \{C. H.\} and Small, \{G. J.\} and R. Jankowiak",

note = "Funding Information: Ames Laborato\textasciitilde{}1 is operated for the US Department of Energy by Iowa State University under contract no. W-7405-Eng-82. This work was supported by the Office of Health and Environmental Research, Office of Energy Research. One of us (C.-H. L.) was supported by the National Institute of Health, grant no. POI CA49210-05. The authors thank Dr. E.L. Cavalieri and Dr. K.-M. Li (Eppley Institute for Research in Cancer, Omaha, NE, USA) for providing us with the DNA-adduct samples.",

year = "1997",

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doi = "10.1016/S0021-9673(97)00499-8",

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AU - Zamzow, D.

AU - Lin, C. H.

AU - Small, G. J.

AU - Jankowiak, R.

N1 - Funding Information: Ames Laborato~1 is operated for the US Department of Energy by Iowa State University under contract no. W-7405-Eng-82. This work was supported by the Office of Health and Environmental Research, Office of Energy Research. One of us (C.-H. L.) was supported by the National Institute of Health, grant no. POI CA49210-05. The authors thank Dr. E.L. Cavalieri and Dr. K.-M. Li (Eppley Institute for Research in Cancer, Omaha, NE, USA) for providing us with the DNA-adduct samples.

PY - 1997/9/26

Y1 - 1997/9/26

N2 - Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.

AB - Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.

KW - DNA

KW - Detection, electrophoresis

KW - Fluorescence line-narrowing spectroscopy

KW - Polynuclear aromatic hydrocarbons

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ER -

Application of capillary electrophoresis-fluorescence line-narrowing spectroscopy for on-line spectral characterization of closely related analytes

Abstract

Keywords

ASJC Scopus subject areas

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