Application of capillary electrophoresis-fluorescence line-narrowing spectroscopy for on-line spectral characterization of closely related analytes

D. Zamzow, C. H. Lin, G. J. Small, R. Jankowiak

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Capillary electrophoresis (CE) interfaced with low-temperature (4.2 K) fluorescence line-narrowing spectroscopy (FLNS) is used for the separation and spectral characterization of closely related analytes. In this paper, the CE-FLNS system is applied to the analysis of a mixture of deuterated and protonated benzo[a]pyrene, a mixture of structurally similar benzo[a]pyrene and benzo[e]pyrene, and mixtures of dibenzo[a,l]pyrene-derived adenine DNA adducts. The CE-FLNS system provides on-line separation and high-resolution spectroscopic identification of CE-separated analytes, via fingerprint structure of vibrationally resolved FLN spectra at 4.2 K. The combination of the separation power of CE and the spectral selectivity of FLNS provide a methodology that has potential to become a powerful tool for molecular analyte characterization. The main applications of the CE-FLNS system, due to its selectivity, should be in the chemical analysis of structurally similar analytes and applications where analyte purity and detailed structural characterization are required.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalJournal of Chromatography A
Volume781
Issue number1-2
DOIs
Publication statusPublished - 1997 Sep 26

Keywords

  • DNA
  • Detection, electrophoresis
  • Fluorescence line-narrowing spectroscopy
  • Polynuclear aromatic hydrocarbons

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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