Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets

Hsien Chun Lin; Mei Hsiang Lin; Jiahn Haur Liao; Tzu Hua Wu; Tzong Huei Lee; Fwu Long Mi; Chi Hao Wu; Ku Chung Chen; Chia Hsiung Cheng; Cheng Wei Lin

doi:10.1021/acs.jafc.6b04101

Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets

Hsien Chun Lin, Mei Hsiang Lin, Jiahn Haur Liao, Tzu Hua Wu, Tzong Huei Lee, Fwu Long Mi, Chi Hao Wu, Ku Chung Chen, Chia Hsiung Cheng, Cheng Wei Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.

Original language	English
Pages (from-to)	51-59
Number of pages	9
Journal	Journal of Agricultural and Food Chemistry
Volume	65
Issue number	1
DOIs	https://doi.org/10.1021/acs.jafc.6b04101
Publication status	Published - 2017 Jan 11
Externally published	Yes

Keywords

Antrodia camphorata
Antroquinonol
Cancer prevention
Colon cancer stem cell
β-catenin

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.jafc.6b04101

Cite this

Lin, H. C., Lin, M. H., Liao, J. H., Wu, T. H., Lee, T. H., Mi, F. L., Wu, C. H., Chen, K. C., Cheng, C. H., & Lin, C. W. (2017). Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets. Journal of Agricultural and Food Chemistry, 65(1), 51-59. https://doi.org/10.1021/acs.jafc.6b04101

Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets. / Lin, Hsien Chun; Lin, Mei Hsiang; Liao, Jiahn Haur et al.
In: Journal of Agricultural and Food Chemistry, Vol. 65, No. 1, 11.01.2017, p. 51-59.

Research output: Contribution to journal › Article › peer-review

Lin, HC, Lin, MH, Liao, JH, Wu, TH, Lee, TH, Mi, FL, Wu, CH, Chen, KC, Cheng, CH & Lin, CW 2017, 'Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets', Journal of Agricultural and Food Chemistry, vol. 65, no. 1, pp. 51-59. https://doi.org/10.1021/acs.jafc.6b04101

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title = "Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets",

abstract = "Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.",

keywords = "Antrodia camphorata, Antroquinonol, Cancer prevention, Colon cancer stem cell, β-catenin",

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year = "2017",

month = jan,

day = "11",

doi = "10.1021/acs.jafc.6b04101",

language = "English",

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T1 - Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties

T2 - Insights into the molecular mechanism & inhibitory targets

AU - Lin, Hsien Chun

AU - Lin, Mei Hsiang

AU - Liao, Jiahn Haur

AU - Wu, Tzu Hua

AU - Lee, Tzong Huei

AU - Mi, Fwu Long

AU - Wu, Chi Hao

AU - Chen, Ku Chung

AU - Cheng, Chia Hsiung

AU - Lin, Cheng Wei

PY - 2017/1/11

Y1 - 2017/1/11

N2 - Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.

AB - Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI50) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 μM. Moreover, ANQ exhibited inhibitory activities toward migration/ invasion and tumorsphere formation of colon cancer cells. Mechanistically, ANQ inhibited pluripotent and cancer stem cellrelated genes and down-regulated β-catenin/T-cell factor (TCF) signaling. Moreover, activation of the phosphatidylinositol-3- kinase (PI3K)/AKT/β-catenin signaling axis was identified to be crucial for regulating the expressions of pluripotent genes, whereas suppression of PI3K/AKT by ANQ inhibited expressions of β-catenin and downstream targets. Molecular docking identified the potential interaction of ANQ with PI3K. Our data show for the first time that the bioactive component of A. camphorata, ANQ, suppresses stem cell-like properties via targeting PI3K/AKT/β-catenin signaling. ANQ could be a promising cancer prevention agent for colon cancer.

KW - Antrodia camphorata

KW - Antroquinonol

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KW - Colon cancer stem cell

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Antroquinonol, a ubiquinone derivative from the mushroom antrodia camphorata, inhibits colon cancer stem cell-like properties: Insights into the molecular mechanism & inhibitory targets

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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