Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats

Yen Hsien Lee, Jung Jhih Chang, Chiang Ting Chien, Ming Chien Yang, Hsiung Fei Chien

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

Original languageEnglish
Article number504693
JournalExperimental Diabetes Research
Volume2012
DOIs
Publication statusPublished - 2012 Sep 18

Fingerprint

UCON 50-HB-5100
Poloxamer
Polymethyl Methacrylate
Streptozocin
Wound Healing
Antioxidants
Ascorbic Acid
Gels
Skin
Wounds and Injuries
Collagen
Apoptosis
Drug Delivery Systems
Healing
Vitamin C
Sol-gel
Rat
Wistar Rats
Air

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Music

Cite this

Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats. / Lee, Yen Hsien; Chang, Jung Jhih; Chien, Chiang Ting; Yang, Ming Chien; Chien, Hsiung Fei.

In: Experimental Diabetes Research, Vol. 2012, 504693, 18.09.2012.

Research output: Contribution to journalArticle

Lee, Yen Hsien ; Chang, Jung Jhih ; Chien, Chiang Ting ; Yang, Ming Chien ; Chien, Hsiung Fei. / Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats. In: Experimental Diabetes Research. 2012 ; Vol. 2012.
@article{911b612a936c4d1bae945cc9702c4760,
title = "Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats",
abstract = "We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.",
author = "Lee, {Yen Hsien} and Chang, {Jung Jhih} and Chien, {Chiang Ting} and Yang, {Ming Chien} and Chien, {Hsiung Fei}",
year = "2012",
month = "9",
day = "18",
doi = "10.1155/2012/504693",
language = "English",
volume = "2012",
journal = "Experimental Diabesity Research",
issn = "1543-8600",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats

AU - Lee, Yen Hsien

AU - Chang, Jung Jhih

AU - Chien, Chiang Ting

AU - Yang, Ming Chien

AU - Chien, Hsiung Fei

PY - 2012/9/18

Y1 - 2012/9/18

N2 - We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

AB - We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H 2 O 2, and O 2 - was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

UR - http://www.scopus.com/inward/record.url?scp=84866163538&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866163538&partnerID=8YFLogxK

U2 - 10.1155/2012/504693

DO - 10.1155/2012/504693

M3 - Article

C2 - 22919368

AN - SCOPUS:84866163538

VL - 2012

JO - Experimental Diabesity Research

JF - Experimental Diabesity Research

SN - 1543-8600

M1 - 504693

ER -