Anti-inflammatory effect of charantadiol a, isolated from wild bitter melon leaf, on heat-inactivated porphyromonas gingivalis-stimulated thp-1 monocytes and a periodontitis mouse model

Tzung Hsun Tsai, Chi I. Chang, Ya Ling Hung, Wen Cheng Huang, Hsiang Chang, Yueh Hsiung Kuo, Jong Ho Chyuan, Lu Te Chuang*, Po Jung Tsai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Porphyromonas gingivalis has been identified as one of the major periodontal pathogens. Activity-directed fractionation and purification processes were employed to identify bioactive compounds from bitter melon leaf. Ethanolic extract of bitter melon leaf was separated into five subfrac-tions by open column chromatography. Subfraction-5-3 significantly inhibited P. gingivalis-induced interleukin (IL)-8 and IL-6 productions in human monocytic THP-1 cells and then was subjected to separation and purification by using different chromatographic methods. Consequently, 5β,19-epoxycucurbita-6,23(E),25(26)-triene-3β,19(R)-diol (charantadiol A) was identified and isolated from the subfraction-5-3. Charantadiol A effectively reduced P. gingivalis-induced IL-6 and IL-8 productions and triggered receptors expressed on myeloid cells (TREM)-1 mRNA level of THP-1 cells. In a separate study, charantadiol A significantly suppressed P. gingivalis-stimulated IL-6 and tumor necrosis factor-α mRNA levels in gingival tissues of mice, confirming the inhibitory effect against P. gingivalis-induced periodontal inflammation. Thus, charantadiol A is a potential anti-inflammatory agent for modulating P. gingivalis-induced inflammation.

Original languageEnglish
Article number5651
JournalMolecules
Volume26
Issue number18
DOIs
Publication statusPublished - 2021 Sep

Keywords

  • Anti-inflammation
  • Bitter melon
  • Charantadiol A
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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