Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish

Yuan Hsiang Lin, Giun Yi Hung, Liang Chun Wu, Sheng Wen Chen, Li Yih Lin, Jiun Lin Horng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The anion exchanger (AE) plays critical roles in physiological processes including CO2 transport and volume regulation in erythrocytes and acid-base regulation in renal tubules. Although expression of the AE in inner-ear hair cells was reported, its specific localization and function are still unclear. Using in situ hybridization, we found that the AE1b transcript is expressed in lateral-line hair cells of zebrafish larvae. An immunohistochemical analysis with a zebrafish-specific antibody localized AE1b to stereocilia of hair cells, and the expression was eliminated by morpholino knockdown of AE1b. A non-invasive, scanning ion-selective electrode technique was applied to analyze mechanotransducer (MET) channelmediated Ca2+ influx at stereocilia of hair cells of intact fish. Ca2+ influx was effectively suppressed by AE1b morpholino knockdown and inhibitor (DIDS) treatment. Elevating external Ca2+ (0.2 to 2 mM) neutralized the inhibition of DIDS. Taken together, this study provides solid evidence to show that AE1b in stereocilia is required for the proper functioning of MET channels.

Original languageEnglish
Article numbere0117041
JournalPloS one
Volume10
Issue number2
DOIs
Publication statusPublished - 2015 Feb 13

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Stereocilia
Zebrafish
Danio rerio
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
hairs
Anions
Morpholinos
Cells
Inner Auditory Hair Cells
calcium
Physiological Phenomena
Ion-Selective Electrodes
specific ion electrodes
Inner Ear
cells
renal tubules
In Situ Hybridization
Larva
Fishes
Erythrocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. / Lin, Yuan Hsiang; Hung, Giun Yi; Wu, Liang Chun; Chen, Sheng Wen; Lin, Li Yih; Horng, Jiun Lin.

In: PloS one, Vol. 10, No. 2, e0117041, 13.02.2015.

Research output: Contribution to journalArticle

Lin, Yuan Hsiang ; Hung, Giun Yi ; Wu, Liang Chun ; Chen, Sheng Wen ; Lin, Li Yih ; Horng, Jiun Lin. / Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. In: PloS one. 2015 ; Vol. 10, No. 2.
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