Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS)

Yao Fang Hsieh; Chih Hsien Chen; Ou Yang Mang; Jeng Ren Duann; Jin Chern Chiou; Shun De Wu; Yong Jiun Lin; Ming Hsui Tsai; Nai Wen Chang

doi:10.1117/12.907930

Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS)

Yao Fang Hsieh^*
, Chih Hsien Chen
, Ou Yang Mang
, Jeng Ren Duann
, Jin Chern Chiou
, Shun De Wu
, Yong Jiun Lin
, Ming Hsui Tsai
, Nai Wen Chang

^*Corresponding author for this work

Department of Mechatronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Currently, the cancer was examined by diagnosing the pathological changes of tumor. If the examination of cancer can diagnose the tumor before the cell occur the pathological changes, the cure rate of cancer will increase. This research develops a human-machine interface for hyper-spectral microscope. The hyper-spectral microscope can scan the specific area of cell and records the data of spectrum and intensity. These data is helpful to diagnose tumor. This study finds the hyper-spectral imaging have two higher intensity points at 550nm and 700nm, and one lower point at 640nm between the two higher points. For analyzing the hyper-spectral imaging, the intensity at the 550nm peak divided by the intensity at 700nm peak. Finally, we determine the accuracy of detection by Gaussian distribution. The accuracy of detecting normal cells achieves 89%, and the accuracy of cancer cells achieves 81%.

Original language	English
Title of host publication	Three-Dimensional and Multidimensional Microscopy
Subtitle of host publication	Image Acquisition and Processing XIX
DOIs	https://doi.org/10.1117/12.907930
Publication status	Published - 2012
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX - San Francisco, CA, United States Duration: 2012 Jan 24 → 2012 Jan 26

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8227
ISSN (Print)	1605-7422

Conference

Conference	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Country/Territory	United States
City	San Francisco, CA
Period	2012/01/24 → 2012/01/26

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cancer
chacteristic
diagnosis
fluorescence
hyperspectral
microscope

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.907930

Cite this

Hsieh, Y. F., Chen, C. H., Mang, O. Y., Duann, J. R., Chiou, J. C., Wu, S. D., Lin, Y. J., Tsai, M. H., & Chang, N. W. (2012). Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS). In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX Article 822712 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8227). https://doi.org/10.1117/12.907930

Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS). / Hsieh, Yao Fang; Chen, Chih Hsien; Mang, Ou Yang et al.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX. 2012. 822712 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8227).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hsieh, YF, Chen, CH, Mang, OY, Duann, JR, Chiou, JC, Wu, SD, Lin, YJ, Tsai, MH & Chang, NW 2012, Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS). in Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX., 822712, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, San Francisco, CA, United States, 2012/01/24. https://doi.org/10.1117/12.907930

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title = "Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS)",

abstract = "Currently, the cancer was examined by diagnosing the pathological changes of tumor. If the examination of cancer can diagnose the tumor before the cell occur the pathological changes, the cure rate of cancer will increase. This research develops a human-machine interface for hyper-spectral microscope. The hyper-spectral microscope can scan the specific area of cell and records the data of spectrum and intensity. These data is helpful to diagnose tumor. This study finds the hyper-spectral imaging have two higher intensity points at 550nm and 700nm, and one lower point at 640nm between the two higher points. For analyzing the hyper-spectral imaging, the intensity at the 550nm peak divided by the intensity at 700nm peak. Finally, we determine the accuracy of detection by Gaussian distribution. The accuracy of detecting normal cells achieves 89\%, and the accuracy of cancer cells achieves 81\%.",

keywords = "cancer, chacteristic, diagnosis, fluorescence, hyperspectral, microscope",

author = "Hsieh, \{Yao Fang\} and Chen, \{Chih Hsien\} and Mang, \{Ou Yang\} and Duann, \{Jeng Ren\} and Chiou, \{Jin Chern\} and Wu, \{Shun De\} and Lin, \{Yong Jiun\} and Tsai, \{Ming Hsui\} and Chang, \{Nai Wen\}",

year = "2012",

doi = "10.1117/12.907930",

language = "English",

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series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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TY - GEN

T1 - Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS)

AU - Hsieh, Yao Fang

AU - Chen, Chih Hsien

AU - Mang, Ou Yang

AU - Duann, Jeng Ren

AU - Chiou, Jin Chern

AU - Wu, Shun De

AU - Lin, Yong Jiun

AU - Tsai, Ming Hsui

AU - Chang, Nai Wen

PY - 2012

Y1 - 2012

N2 - Currently, the cancer was examined by diagnosing the pathological changes of tumor. If the examination of cancer can diagnose the tumor before the cell occur the pathological changes, the cure rate of cancer will increase. This research develops a human-machine interface for hyper-spectral microscope. The hyper-spectral microscope can scan the specific area of cell and records the data of spectrum and intensity. These data is helpful to diagnose tumor. This study finds the hyper-spectral imaging have two higher intensity points at 550nm and 700nm, and one lower point at 640nm between the two higher points. For analyzing the hyper-spectral imaging, the intensity at the 550nm peak divided by the intensity at 700nm peak. Finally, we determine the accuracy of detection by Gaussian distribution. The accuracy of detecting normal cells achieves 89%, and the accuracy of cancer cells achieves 81%.

AB - Currently, the cancer was examined by diagnosing the pathological changes of tumor. If the examination of cancer can diagnose the tumor before the cell occur the pathological changes, the cure rate of cancer will increase. This research develops a human-machine interface for hyper-spectral microscope. The hyper-spectral microscope can scan the specific area of cell and records the data of spectrum and intensity. These data is helpful to diagnose tumor. This study finds the hyper-spectral imaging have two higher intensity points at 550nm and 700nm, and one lower point at 640nm between the two higher points. For analyzing the hyper-spectral imaging, the intensity at the 550nm peak divided by the intensity at 700nm peak. Finally, we determine the accuracy of detection by Gaussian distribution. The accuracy of detecting normal cells achieves 89%, and the accuracy of cancer cells achieves 81%.

KW - cancer

KW - chacteristic

KW - diagnosis

KW - fluorescence

KW - hyperspectral

KW - microscope

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UR - https://www.scopus.com/pages/publications/84859613208#tab=citedBy

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DO - 10.1117/12.907930

M3 - Conference contribution

AN - SCOPUS:84859613208

SN - 9780819488701

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Three-Dimensional and Multidimensional Microscopy

T2 - Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX

Y2 - 24 January 2012 through 26 January 2012

ER -

Analyze fluorescent characteristic of cancer cell using hyperspectroscopic imaging system (HIS)

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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