Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR

Guan Chiun Lee, Shye Jye Tang, Kuang Hui Sun, Jei Fu Shaw

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Synthesis of multiple extracellular lipases in Candida rugosa has been demonstrated. However, it is difficult to characterize the expression spectrum of lip genes, since the sequences of the lip multigene family are very closely related. A competitive reverse transcription-PCR assay was developed to quantify the expression of lip genes. In agreement with the protein profile, the abundance of lip mRNAs was found to be (in decreasing order) lip1, lip3, lip2, lip5, and lip4. To analyze the effects of different culture conditions, the transcript concentrations for these mRNA species were normalized relative to the values for gpd, encoding glyceraldehyde-3- phosphate dehydrogenase. In relative terms, lip1 and lip3 were highly and constitutively expressed (about 105 molecules per μg of total RNA) whereas the other inducible lip genes, especially lip4, showed significant changes in mRNA expression under different culture conditions. These results indicate that differential transcriptional control of lip genes results in multiple forms of lipase proteins.

Original languageEnglish
Pages (from-to)3888-3895
Number of pages8
JournalApplied and Environmental Microbiology
Volume65
Issue number9
DOIs
Publication statusPublished - 1999 Sep

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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