TY - JOUR
T1 - Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique
AU - Su, An Kai
AU - Chang, Yen Sheng
AU - Lin, Cheng Huang
N1 - Funding Information:
This work was supported by a grant from the National Science Council of Taiwan under Contract no. NSC-90-2113-M-003-020.
PY - 2004/11/15
Y1 - 2004/11/15
N2 - A simple, inexpensive and reliable method for the routine analysis of riboflavin in beer by capillary electrophoresis-light emitting diode (CE-LED) induced fluorescence detection is described. A simple and straightforward sample preparation is involved and the method is based on an inexpensive blue LED as the light source combined with an on-line sample concentration technique. For this detection system, using a normal micellar electrokinetic chromatography (MEKC), stacking-MEKC and dynamic pH junction techniques, the detection limits were found to be 480, 20 and 1 ng mL-1, respectively. In addition, the number of theoretical plates for riboflavin was determined to be 3.8×104 by means of a dynamic pH junction and this was improved to 3.2×106 when the dynamic pH junction-sweeping mode was applied. The concentrations of riboflavin in 12 samples of different types of commercial beer were found to be in the range of 130-280 ng mL-1.
AB - A simple, inexpensive and reliable method for the routine analysis of riboflavin in beer by capillary electrophoresis-light emitting diode (CE-LED) induced fluorescence detection is described. A simple and straightforward sample preparation is involved and the method is based on an inexpensive blue LED as the light source combined with an on-line sample concentration technique. For this detection system, using a normal micellar electrokinetic chromatography (MEKC), stacking-MEKC and dynamic pH junction techniques, the detection limits were found to be 480, 20 and 1 ng mL-1, respectively. In addition, the number of theoretical plates for riboflavin was determined to be 3.8×104 by means of a dynamic pH junction and this was improved to 3.2×106 when the dynamic pH junction-sweeping mode was applied. The concentrations of riboflavin in 12 samples of different types of commercial beer were found to be in the range of 130-280 ng mL-1.
KW - Blue light emitting diode (LED)
KW - CE
KW - Capillary electrophoresis
KW - Dynamic pH junction
KW - Dynamic pH junction-sweeping
KW - LED
KW - Riboflavin
KW - SDS
KW - capillary electrophoresis
KW - light emitting diode
KW - sodium dodecyl sulfate
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U2 - 10.1016/j.talanta.2004.04.011
DO - 10.1016/j.talanta.2004.04.011
M3 - Article
AN - SCOPUS:5144219972
SN - 0039-9140
VL - 64
SP - 970
EP - 974
JO - Talanta
JF - Talanta
IS - 4
ER -