Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique

An Kai Su, Yen Sheng Chang, Cheng Huang Lin

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

A simple, inexpensive and reliable method for the routine analysis of riboflavin in beer by capillary electrophoresis-light emitting diode (CE-LED) induced fluorescence detection is described. A simple and straightforward sample preparation is involved and the method is based on an inexpensive blue LED as the light source combined with an on-line sample concentration technique. For this detection system, using a normal micellar electrokinetic chromatography (MEKC), stacking-MEKC and dynamic pH junction techniques, the detection limits were found to be 480, 20 and 1 ng mL-1, respectively. In addition, the number of theoretical plates for riboflavin was determined to be 3.8×104 by means of a dynamic pH junction and this was improved to 3.2×106 when the dynamic pH junction-sweeping mode was applied. The concentrations of riboflavin in 12 samples of different types of commercial beer were found to be in the range of 130-280 ng mL-1.

Original languageEnglish
Pages (from-to)970-974
Number of pages5
JournalTalanta
Volume64
Issue number4
DOIs
Publication statusPublished - 2004 Nov 15

Keywords

  • Blue light emitting diode (LED)
  • CE
  • Capillary electrophoresis
  • Dynamic pH junction
  • Dynamic pH junction-sweeping
  • LED
  • Riboflavin
  • SDS
  • capillary electrophoresis
  • light emitting diode
  • sodium dodecyl sulfate

ASJC Scopus subject areas

  • Analytical Chemistry

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