Analysis of heat-shock protein 70 gene polymorphisms and the risk of Parkinson's disease

Yih Ru Wu, Cheng Kuang Wang, Chiung Mei Chen, Yuying Hsu, Sih Jing Lin, Yi Ying Lin, Hon Chung Fung, Kuo Hsuan Chang, Guey Jen Lee-Chen

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110 Citations (Scopus)

Abstract

Parkinson's disease (PD) involves several genetic and environmental components. Heat-shock protein 70, a chaperone that is up-regulated in stress responses and that refolds protein, may be involved in the pathogenesis of PD. We have investigated the association of polymorphisms -110 A/C, +190 G/C, +1267 A/G, +2074 G/C, and +2437 G/C in the 5′ and coding regions of the HSP70-1, HSP70-2, and HSP70-hom genes with the risk of PD by screening DNA samples from 274 PD patients and 183 controls in assays based on the polymerase chain reaction. There was no statistically significant difference in genotype distribution between patients and controls for the three coding-region polymorphisms in HSP70-2 and HSP70-hom. However, for HSP70-1, the overall genotype distribution was significantly different at the -110 site (P=0.004) and tended to be different at the +190 site (P= 0.012) between patients and controls. The frequencies of the -110 CC and +190 CC genotypes were significantly higher in PD patients than in controls (P=0.001 and 0.006, respectively). Both -110 CC (odds ratio: 2.91; 95% CI: 1.51-5.96; P=0.002) and +190 CC (odds ratio: 3.59; 95% CI: 1.53-9.88; P=0.006) genotypes were significantly associated with PD. Reporter constructs containing the -110 A allele cloned into a luciferase reporter plasmid drove marginally higher transcriptional activity of HSP70-1 compared with the -110 C allele in both control and heat-shocked IMR32 and 293 cells. Therefore, -110 A/C may be a functional polymorphism in the 5′ promoter region of HSP70-1 and may affect susceptibility to PD.

Original languageEnglish
Pages (from-to)236-241
Number of pages6
JournalHuman Genetics
Volume114
Issue number3
DOIs
Publication statusPublished - 2004 Feb 1

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Wu, Y. R., Wang, C. K., Chen, C. M., Hsu, Y., Lin, S. J., Lin, Y. Y., Fung, H. C., Chang, K. H., & Lee-Chen, G. J. (2004). Analysis of heat-shock protein 70 gene polymorphisms and the risk of Parkinson's disease. Human Genetics, 114(3), 236-241. https://doi.org/10.1007/s00439-003-1050-1