TY - JOUR
T1 - Analysis of gene expression in single human oocytes and preimplantation embryos
AU - Li, Steven Shoei Lung
AU - Liu, Yung Hsien
AU - Tseng, Chao Neng
AU - Singh, Sher
N1 - Funding Information:
We thank Miss Tzi-Yi Lee, Mr. Tung-Liang Chung, Miss Ya-Lan Hsien, Miss Jing-Yi Shen, and Dr. Hong-Lin Su for their helps during this investigation. The cDNA microarray experiments were carried out by the DNA microarray core facility for Genomic Medicine at National Taiwan University. This investigation was supported in part by a grant from the National Genomic Medicine Program of the National Science Council of Taiwan to S.S.-L. Li, who is also supported by the Foundation for the Development of Outstanding Scholarship in Taiwan and a Chair Professorship of the Medical Education and Development Foundation of Kaohsiung Medical University.
PY - 2006/2/3
Y1 - 2006/2/3
N2 - Little is known about the gene expression in human oocytes and early embryo development because of the rare availability of the materials. The recent advancement of biotechnology has allowed one to analyze the gene expression in single human oocytes and preimplantation embryos. Gene expression of human lactate dehydrogenase isozymes (LDH-A, LDH-B, and LDH-C) and small ubiquitin-like modifier isoforms (SUMO-1, SUMO-2, and SUMO-3) in four oocytes, two 4-cell and three 8-cell embryos was studied using the reverse transcription-polymerase chain reaction. The mRNAs for SUMO-1, SUMO-2, SUMO-3, and LDH-B (heart) were detected in all of oocytes, 4- and 8-cell embryos. The mRNA for LDH-A (muscle) was detected in two of four oocytes and one of three 8-cell embryos. However, the mRNA for testis-specific LDH-C was not detected at all as expected. A cDNA microarray containing 9600 cDNA spots was used to investigate differential expression of human genes in oocyte, 4-cell and 8-cell embryos. The expression of 184, 29, and 65 genes was found to have a value more than twofold above the median value of all genes expressed in oocyte, 4- and 8-cell embryos, respectively, indicating that the expression of some zygotic genes had already occurred at 4-cell embryo.
AB - Little is known about the gene expression in human oocytes and early embryo development because of the rare availability of the materials. The recent advancement of biotechnology has allowed one to analyze the gene expression in single human oocytes and preimplantation embryos. Gene expression of human lactate dehydrogenase isozymes (LDH-A, LDH-B, and LDH-C) and small ubiquitin-like modifier isoforms (SUMO-1, SUMO-2, and SUMO-3) in four oocytes, two 4-cell and three 8-cell embryos was studied using the reverse transcription-polymerase chain reaction. The mRNAs for SUMO-1, SUMO-2, SUMO-3, and LDH-B (heart) were detected in all of oocytes, 4- and 8-cell embryos. The mRNA for LDH-A (muscle) was detected in two of four oocytes and one of three 8-cell embryos. However, the mRNA for testis-specific LDH-C was not detected at all as expected. A cDNA microarray containing 9600 cDNA spots was used to investigate differential expression of human genes in oocyte, 4-cell and 8-cell embryos. The expression of 184, 29, and 65 genes was found to have a value more than twofold above the median value of all genes expressed in oocyte, 4- and 8-cell embryos, respectively, indicating that the expression of some zygotic genes had already occurred at 4-cell embryo.
KW - Human
KW - Oocytes
KW - Preimplantation embryos
KW - RT-PCR
KW - cDNA microarray
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U2 - 10.1016/j.bbrc.2005.11.149
DO - 10.1016/j.bbrc.2005.11.149
M3 - Article
C2 - 16343430
AN - SCOPUS:29244455294
SN - 0006-291X
VL - 340
SP - 48
EP - 53
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -