An experimental model to evaluate the in vivo response of rat seminal vesicle to electrical stimulation

Ju Ton Hsieh; Chiang Ting Chien; Shih Ping Liu; Chau Fong Chen; Ming Kuen Lai

doi:10.1016/0304-3940(96)12354-5

An experimental model to evaluate the in vivo response of rat seminal vesicle to electrical stimulation

Ju Ton Hsieh^*, Chiang Ting Chien, Shih Ping Liu, Chau Fong Chen, Ming Kuen Lai

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

This investigation demonstrated a good model to record the pressure change of rat seminal vesicle (SV) in response to electrical stimulation (ES) of lesser splanchnic nerve (LSN) in vivo. LSN was easily identified and isolated grossly. Antegrade catheterization via the SV tail to the main lumen was performed similar to the technique of i.v. catheterization, and the pressure recording was quite satisfactory. The vesicle response to ES was frequency-dependent and reproducible. Under the conditions of 10 V and 1 ms, the maximal vesicle pressure was 81.7 ± 2.8 mmHg with 80 Hz of ES. The inhibitory response of SV to ES by clomipramine was concentration-dependent. Clomipramine (3 mg/kg) could inhibit 53% of control responses (n = 7). This experimental model is relevant to neurophysiologic and neuropharmacologic studies of SV.

Original language	English
Pages (from-to)	215-217
Number of pages	3
Journal	Neuroscience Letters
Volume	204
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(96)12354-5
Publication status	Published - 1996 Feb 9
Externally published	Yes

Keywords

Clomipramine
Electrical stimulation
In vivo
Lesser splanchnic nerve
Seminal vesicle
Wistar rats

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(96)12354-5

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title = "An experimental model to evaluate the in vivo response of rat seminal vesicle to electrical stimulation",

abstract = "This investigation demonstrated a good model to record the pressure change of rat seminal vesicle (SV) in response to electrical stimulation (ES) of lesser splanchnic nerve (LSN) in vivo. LSN was easily identified and isolated grossly. Antegrade catheterization via the SV tail to the main lumen was performed similar to the technique of i.v. catheterization, and the pressure recording was quite satisfactory. The vesicle response to ES was frequency-dependent and reproducible. Under the conditions of 10 V and 1 ms, the maximal vesicle pressure was 81.7 ± 2.8 mmHg with 80 Hz of ES. The inhibitory response of SV to ES by clomipramine was concentration-dependent. Clomipramine (3 mg/kg) could inhibit 53% of control responses (n = 7). This experimental model is relevant to neurophysiologic and neuropharmacologic studies of SV.",

keywords = "Clomipramine, Electrical stimulation, In vivo, Lesser splanchnic nerve, Seminal vesicle, Wistar rats",

author = "Hsieh, {Ju Ton} and Chien, {Chiang Ting} and Liu, {Shih Ping} and Chen, {Chau Fong} and Lai, {Ming Kuen}",

note = "Funding Information: This study was supported by the National Science Council Grant NSC 85-2331-B-002-032.",

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doi = "10.1016/0304-3940(96)12354-5",

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T1 - An experimental model to evaluate the in vivo response of rat seminal vesicle to electrical stimulation

AU - Hsieh, Ju Ton

AU - Chien, Chiang Ting

AU - Liu, Shih Ping

AU - Chen, Chau Fong

AU - Lai, Ming Kuen

N1 - Funding Information: This study was supported by the National Science Council Grant NSC 85-2331-B-002-032.

PY - 1996/2/9

Y1 - 1996/2/9

N2 - This investigation demonstrated a good model to record the pressure change of rat seminal vesicle (SV) in response to electrical stimulation (ES) of lesser splanchnic nerve (LSN) in vivo. LSN was easily identified and isolated grossly. Antegrade catheterization via the SV tail to the main lumen was performed similar to the technique of i.v. catheterization, and the pressure recording was quite satisfactory. The vesicle response to ES was frequency-dependent and reproducible. Under the conditions of 10 V and 1 ms, the maximal vesicle pressure was 81.7 ± 2.8 mmHg with 80 Hz of ES. The inhibitory response of SV to ES by clomipramine was concentration-dependent. Clomipramine (3 mg/kg) could inhibit 53% of control responses (n = 7). This experimental model is relevant to neurophysiologic and neuropharmacologic studies of SV.

AB - This investigation demonstrated a good model to record the pressure change of rat seminal vesicle (SV) in response to electrical stimulation (ES) of lesser splanchnic nerve (LSN) in vivo. LSN was easily identified and isolated grossly. Antegrade catheterization via the SV tail to the main lumen was performed similar to the technique of i.v. catheterization, and the pressure recording was quite satisfactory. The vesicle response to ES was frequency-dependent and reproducible. Under the conditions of 10 V and 1 ms, the maximal vesicle pressure was 81.7 ± 2.8 mmHg with 80 Hz of ES. The inhibitory response of SV to ES by clomipramine was concentration-dependent. Clomipramine (3 mg/kg) could inhibit 53% of control responses (n = 7). This experimental model is relevant to neurophysiologic and neuropharmacologic studies of SV.

KW - Clomipramine

KW - Electrical stimulation

KW - In vivo

KW - Lesser splanchnic nerve

KW - Seminal vesicle

KW - Wistar rats

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AN - SCOPUS:0030047595

SN - 0304-3940

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EP - 217

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

An experimental model to evaluate the in vivo response of rat seminal vesicle to electrical stimulation

Abstract

Keywords

ASJC Scopus subject areas

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