TY - JOUR
T1 - An approach for differentiating uniform glutamatergic neurons from mouse embryonic stem cells
AU - Chuang, Jen Hua
AU - Tung, Li Chu
AU - Lee-Chen, Guey Jen
AU - Yin, Yi
AU - Lin, Yenshou
PY - 2011/3/1
Y1 - 2011/3/1
N2 - Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Here we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA (ethylenediaminetetraacetic acid) solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, more than 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons that were differentiated from ES cells provide an ideal cellular model for many aspects of research.
AB - Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Here we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA (ethylenediaminetetraacetic acid) solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, more than 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons that were differentiated from ES cells provide an ideal cellular model for many aspects of research.
UR - http://www.scopus.com/inward/record.url?scp=78651352351&partnerID=8YFLogxK
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U2 - 10.1016/j.ab.2010.11.027
DO - 10.1016/j.ab.2010.11.027
M3 - Article
C2 - 21094123
AN - SCOPUS:78651352351
SN - 0003-2697
VL - 410
SP - 149
EP - 151
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -