An approach for differentiating uniform glutamatergic neurons from mouse embryonic stem cells

Jen H. Chuang, Li C. Tung, Guey J. Lee-Chen, Yi Yin, Yenshou Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Here we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA (ethylenediaminetetraacetic acid) solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, more than 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons that were differentiated from ES cells provide an ideal cellular model for many aspects of research.

Original languageEnglish
Pages (from-to)149-151
Number of pages3
JournalAnalytical Biochemistry
Volume410
Issue number1
DOIs
Publication statusPublished - 2011 Mar 1

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Embryonic Stem Cells
Stem cells
Edetic Acid
Neurons
Embryoid Bodies
Trypsin
Research
Population
Mouse Embryonic Stem Cells
carbosulfan

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

An approach for differentiating uniform glutamatergic neurons from mouse embryonic stem cells. / Chuang, Jen H.; Tung, Li C.; Lee-Chen, Guey J.; Yin, Yi; Lin, Yenshou.

In: Analytical Biochemistry, Vol. 410, No. 1, 01.03.2011, p. 149-151.

Research output: Contribution to journalArticle

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