An approach for differentiating uniform glutamatergic neurons from mouse embryonic stem cells

Jen H. Chuang, Li C. Tung, Guey J. Lee-Chen, Yi Yin, Yenshou Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Here we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA (ethylenediaminetetraacetic acid) solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, more than 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons that were differentiated from ES cells provide an ideal cellular model for many aspects of research.

Original languageEnglish
Pages (from-to)149-151
Number of pages3
JournalAnalytical Biochemistry
Volume410
Issue number1
DOIs
Publication statusPublished - 2011 Mar 1

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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