Altered expression of HSPA5, HSPA8 and PARK7 in spinocerebellar ataxia type 17 identified by 2-dimensional fluorescence difference in gel electrophoresis

Li Ching Lee, Chiung Mei Chen, Fen Lin Chen, Pei Ying Lin, Ya Chin Hsiao, Pin Rong Wang, Ming-Tsan Su, Hsiu Mei Hsieh, Ji-Chuu Hwang, Chung-Hsin Wu, Guan-Chiun Lee, Sher Singh, Yen-Shou Lin, Sen Yung Hsieh, Guey-Jen Lee, Jung Yaw Lin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Expansion of the CAG repeat of the TATA-box binding protein (TBP) gene has been identified as the causative mutations in spinocerebellar ataxia 17 (SCA17). TBP is ubiquitously expressed in both central nervous system and peripheral tissues. The underlying molecular changes of SCA17 are rarely explored. Methods: To study the molecular mechanisms underlying SCA17, we generated stably induced isogenic 293 cells expressing normal TBP-Q36 and expanded TBP-Q61 and analyzed the expressed proteins using two-dimensional difference in gel electrophoresis (2D-DIGE), followed by mass spectrometry and immunoblotting. Results: Upon induction with doxycycline, the expanded TBP-Q61 formed aggregates with significant increase in the cell population at subG1 phase and cleaved caspase-3. Proteomics study identified a total of 16 proteins with expression changes greater than 1.5 fold. Among the 16 proteins, PARK7, GLRX3, HNRNPA1, GINS1, ENO1, HNRPK and NPM1 are increased, and SERPINA5, HSPA5, VCL, KHSRP, HSPA8, HNRPH1, IMMT, VCP and HNRNPL are decreased in cells expressing TBP-Q61 compared with those expressing TBP-Q36. The altered expression of HSPA5, HSPA8 and PARK7 were further validated by 2D and Western immunoblot analyses. Conclusions: The results illustrate the utility of proteomics to identify alterations of proteins which underlie pathogenesis of SCA17, and may serve as potential therapeutic targets.

Original languageEnglish
Pages (from-to)56-62
Number of pages7
JournalClinica Chimica Acta
Volume400
Issue number1-2
DOIs
Publication statusPublished - 2009 Feb 1

Fingerprint

TATA-Box Binding Protein
Electrophoresis
Fluorescence
Gels
Two-Dimensional Difference Gel Electrophoresis
Cells
Proteomics
Proteins
Doxycycline
Neurology
Spinocerebellar Ataxia 17
Immunoblotting
Caspase 3
Mass spectrometry
Mass Spectrometry
Central Nervous System
Genes
Western Blotting
Tissue
Mutation

Keywords

  • 2D-DIGE
  • HSPA5
  • HSPA8
  • PARK7
  • Spinocerebellar ataxia type 17
  • TBP expansion

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Altered expression of HSPA5, HSPA8 and PARK7 in spinocerebellar ataxia type 17 identified by 2-dimensional fluorescence difference in gel electrophoresis. / Lee, Li Ching; Chen, Chiung Mei; Chen, Fen Lin; Lin, Pei Ying; Hsiao, Ya Chin; Wang, Pin Rong; Su, Ming-Tsan; Hsieh, Hsiu Mei; Hwang, Ji-Chuu; Wu, Chung-Hsin; Lee, Guan-Chiun; Singh, Sher; Lin, Yen-Shou; Hsieh, Sen Yung; Lee, Guey-Jen; Lin, Jung Yaw.

In: Clinica Chimica Acta, Vol. 400, No. 1-2, 01.02.2009, p. 56-62.

Research output: Contribution to journalArticle

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title = "Altered expression of HSPA5, HSPA8 and PARK7 in spinocerebellar ataxia type 17 identified by 2-dimensional fluorescence difference in gel electrophoresis",
abstract = "Background: Expansion of the CAG repeat of the TATA-box binding protein (TBP) gene has been identified as the causative mutations in spinocerebellar ataxia 17 (SCA17). TBP is ubiquitously expressed in both central nervous system and peripheral tissues. The underlying molecular changes of SCA17 are rarely explored. Methods: To study the molecular mechanisms underlying SCA17, we generated stably induced isogenic 293 cells expressing normal TBP-Q36 and expanded TBP-Q61 and analyzed the expressed proteins using two-dimensional difference in gel electrophoresis (2D-DIGE), followed by mass spectrometry and immunoblotting. Results: Upon induction with doxycycline, the expanded TBP-Q61 formed aggregates with significant increase in the cell population at subG1 phase and cleaved caspase-3. Proteomics study identified a total of 16 proteins with expression changes greater than 1.5 fold. Among the 16 proteins, PARK7, GLRX3, HNRNPA1, GINS1, ENO1, HNRPK and NPM1 are increased, and SERPINA5, HSPA5, VCL, KHSRP, HSPA8, HNRPH1, IMMT, VCP and HNRNPL are decreased in cells expressing TBP-Q61 compared with those expressing TBP-Q36. The altered expression of HSPA5, HSPA8 and PARK7 were further validated by 2D and Western immunoblot analyses. Conclusions: The results illustrate the utility of proteomics to identify alterations of proteins which underlie pathogenesis of SCA17, and may serve as potential therapeutic targets.",
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T1 - Altered expression of HSPA5, HSPA8 and PARK7 in spinocerebellar ataxia type 17 identified by 2-dimensional fluorescence difference in gel electrophoresis

AU - Lee, Li Ching

AU - Chen, Chiung Mei

AU - Chen, Fen Lin

AU - Lin, Pei Ying

AU - Hsiao, Ya Chin

AU - Wang, Pin Rong

AU - Su, Ming-Tsan

AU - Hsieh, Hsiu Mei

AU - Hwang, Ji-Chuu

AU - Wu, Chung-Hsin

AU - Lee, Guan-Chiun

AU - Singh, Sher

AU - Lin, Yen-Shou

AU - Hsieh, Sen Yung

AU - Lee, Guey-Jen

AU - Lin, Jung Yaw

PY - 2009/2/1

Y1 - 2009/2/1

N2 - Background: Expansion of the CAG repeat of the TATA-box binding protein (TBP) gene has been identified as the causative mutations in spinocerebellar ataxia 17 (SCA17). TBP is ubiquitously expressed in both central nervous system and peripheral tissues. The underlying molecular changes of SCA17 are rarely explored. Methods: To study the molecular mechanisms underlying SCA17, we generated stably induced isogenic 293 cells expressing normal TBP-Q36 and expanded TBP-Q61 and analyzed the expressed proteins using two-dimensional difference in gel electrophoresis (2D-DIGE), followed by mass spectrometry and immunoblotting. Results: Upon induction with doxycycline, the expanded TBP-Q61 formed aggregates with significant increase in the cell population at subG1 phase and cleaved caspase-3. Proteomics study identified a total of 16 proteins with expression changes greater than 1.5 fold. Among the 16 proteins, PARK7, GLRX3, HNRNPA1, GINS1, ENO1, HNRPK and NPM1 are increased, and SERPINA5, HSPA5, VCL, KHSRP, HSPA8, HNRPH1, IMMT, VCP and HNRNPL are decreased in cells expressing TBP-Q61 compared with those expressing TBP-Q36. The altered expression of HSPA5, HSPA8 and PARK7 were further validated by 2D and Western immunoblot analyses. Conclusions: The results illustrate the utility of proteomics to identify alterations of proteins which underlie pathogenesis of SCA17, and may serve as potential therapeutic targets.

AB - Background: Expansion of the CAG repeat of the TATA-box binding protein (TBP) gene has been identified as the causative mutations in spinocerebellar ataxia 17 (SCA17). TBP is ubiquitously expressed in both central nervous system and peripheral tissues. The underlying molecular changes of SCA17 are rarely explored. Methods: To study the molecular mechanisms underlying SCA17, we generated stably induced isogenic 293 cells expressing normal TBP-Q36 and expanded TBP-Q61 and analyzed the expressed proteins using two-dimensional difference in gel electrophoresis (2D-DIGE), followed by mass spectrometry and immunoblotting. Results: Upon induction with doxycycline, the expanded TBP-Q61 formed aggregates with significant increase in the cell population at subG1 phase and cleaved caspase-3. Proteomics study identified a total of 16 proteins with expression changes greater than 1.5 fold. Among the 16 proteins, PARK7, GLRX3, HNRNPA1, GINS1, ENO1, HNRPK and NPM1 are increased, and SERPINA5, HSPA5, VCL, KHSRP, HSPA8, HNRPH1, IMMT, VCP and HNRNPL are decreased in cells expressing TBP-Q61 compared with those expressing TBP-Q36. The altered expression of HSPA5, HSPA8 and PARK7 were further validated by 2D and Western immunoblot analyses. Conclusions: The results illustrate the utility of proteomics to identify alterations of proteins which underlie pathogenesis of SCA17, and may serve as potential therapeutic targets.

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KW - HSPA8

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KW - TBP expansion

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