Alterations of nPKC distribution, but normal Akt/PKB activation in denervated rat soleus muscle

Yenshou Lin, Matthew J. Brady, Kristen Wolanske, Richard Holbert, Neil B. Ruderman, Gordon C. Yaney

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Denervation has been shown to impair the ability of insulin to stimulate glycogen synthesis and, to a lesser extent, glucose transport in rat skeletal muscle. Insulin binding to its receptor, activation of the receptor tyrosine kinase and phosphatidylinositol 3′-kinase do not appear to be involved. On the other hand, it has been shown that denervation causes an increase in the total diacylglycerol (DAG) content and membrane-associated protein kinase C (PKC) activity. In this study, we further characterize these changes in PKC and assess other possible signaling abnormalities that might be related to the decrease of glycogen synthesis. The results reveal that PKC-ε and -θ, but not -α or are increased in the membrane fraction 24 h after denervation and that the timing of these changes parallels the impaired ability of insulin to stimulate glycogen synthesis. At 24 h, these changes were associated with a 65% decrease in glycogen synthase (GS) activity ratio and decreased electrophoretic mobility, indicative of phosphorylation in GS in muscles incubated in the absence of insulin. Incubation of the denervated soleus with insulin for 30 min minimally increased glucose incorporation into glycogen; however, it increased GS activity threefold, to a value still less than that of control muscle, and it eliminated the gel shift. In addition, insulin increased the apparent abundance of GS kinase (GSK)-3 and protein phosphatase (PP)1α in the supernatant fraction of muscle homogenate to control values, and it caused the same increases in GSK-3 and Akt/protein kinase B (PKB) phosphorylation and Akt/PKB activity that it did in nondenervated muscle. No alterations in hexokinase I or II activity were observed after denervation; however, in agreement with a previous report, glucose 6-phosphate levels were diminished in 24-h-denervated soleus, and they did not increase after insulin stimulation. These results indicate that alterations in the distribution of PKC-ε and -θ accompany the impairment of glycogen synthesis in the 24-h-denervated soleus. They also indicate that the basal rate of glycogen synthesis and its stimulation by insulin in these muscles are diminished despite a normal activation of Akt/PKB and phosphorylation of GSK-3. The significance of the observed alterations to GSK-3 and PP1α distribution remain to be determined.

Original languageEnglish
Pages (from-to)E318-E325
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume283
Issue number2 46-2
Publication statusPublished - 2002 Aug 10

Fingerprint

Proto-Oncogene Proteins c-akt
Normal Distribution
Normal distribution
Muscle
Rats
Skeletal Muscle
Chemical activation
Glycogen
Insulin
Denervation
Glycogen Synthase
Protein Kinase C
Phosphorylation
Muscles
Phosphotransferases
Phosphatidylinositol 3-Kinase
Glycogen Synthase Kinase 3
Protein Phosphatase 1
Glucose
Electrophoretic mobility

Keywords

  • Akt/protein kinase B
  • Denervation
  • Glycogen synthase
  • Glycogen synthase kinase 3
  • Novel protein kinase C
  • Protein phosphatase-1
  • Soleus muscle

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Lin, Y., Brady, M. J., Wolanske, K., Holbert, R., Ruderman, N. B., & Yaney, G. C. (2002). Alterations of nPKC distribution, but normal Akt/PKB activation in denervated rat soleus muscle. American Journal of Physiology - Endocrinology and Metabolism, 283(2 46-2), E318-E325.

Alterations of nPKC distribution, but normal Akt/PKB activation in denervated rat soleus muscle. / Lin, Yenshou; Brady, Matthew J.; Wolanske, Kristen; Holbert, Richard; Ruderman, Neil B.; Yaney, Gordon C.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 283, No. 2 46-2, 10.08.2002, p. E318-E325.

Research output: Contribution to journalArticle

Lin, Y, Brady, MJ, Wolanske, K, Holbert, R, Ruderman, NB & Yaney, GC 2002, 'Alterations of nPKC distribution, but normal Akt/PKB activation in denervated rat soleus muscle', American Journal of Physiology - Endocrinology and Metabolism, vol. 283, no. 2 46-2, pp. E318-E325.
Lin, Yenshou ; Brady, Matthew J. ; Wolanske, Kristen ; Holbert, Richard ; Ruderman, Neil B. ; Yaney, Gordon C. / Alterations of nPKC distribution, but normal Akt/PKB activation in denervated rat soleus muscle. In: American Journal of Physiology - Endocrinology and Metabolism. 2002 ; Vol. 283, No. 2 46-2. pp. E318-E325.
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