Alterations in peripheral purinergic and muscarinic signaling of rat bladder after long-term fructose-induced metabolic syndrome

Shiu Dong Chung, Chiang Ting Chien, Hong Jeng Yu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: We explored the pathophysiologic mechanisms of long-term fructose-induced lower urinary tract symptoms (LUTS) in rats. Methods: Male Wistar rats were fed with fructose for 3 or 6 months. Biochemical and transcystometric parameters were compared between fructose-fed and age-matched normal-diet rats. Pelvic nerve and external urethral sphincter-electromyogram activity recordings were performed to investigate fructose effects on neural control of bladders. Mitochondrial structure, ATP and acetylcholine content and purinergic and muscarinic cholinergic receptors were examined. Cytosolic cytochrome C staining by Western blot and immunocytochemistry for mitochondrial injury and PGP 9.5 stain for nerve density were also determined. Results: The fructose-fed rats with higher plasma triglyceride, LDL and fasting glucose levels displayed LUTS with increased frequency and suppressed voiding contractile amplitude in phase 1 and phase 2 duration versus normal-diet control. Fructose feeding altered the firing types in pelvic afferent and efferent nerves and external urethral sphincter-electromyogram activity. Increased mast cell number, disrupted and swollen mitochondria, increased cytosolic cytochrome C stain and expression and decreased nerve density in bladder smooth muscle layers appeared in the fructose-fed rats. Fructose feeding also significantly reduced ATP and acetylcholine content and enhanced protein expression of postsynaptic P2X1, P2X 2 and P2X3 purinergic receptors and M 2 and M3 muscarinic cholinergic receptors expression in the smooth muscles of urinary bladder. Conclusion: Long-term fructose feeding induced neuropathy and myopathy in the urinary bladders. Impaired mitochondrial integrity, reduced nerve density, ATP and acetylcholine content and upregulation of purinergic and muscarinic cholinergic receptors expression may contribute to the bladder dysfunction of fructose-fed animals.

Original languageEnglish
Pages (from-to)347-359
Number of pages13
JournalEuropean Journal of Nutrition
Volume52
Issue number1
DOIs
Publication statusPublished - 2013 Feb 1

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Fructose
Cholinergic Agents
Urinary Bladder
Cholinergic Receptors
Muscarinic Receptors
Acetylcholine
Lower Urinary Tract Symptoms
Adenosine Triphosphate
Electromyography
Urethra
Cytochromes
Smooth Muscle
Purinergic P2X Receptors
Coloring Agents
Purinergic P2X3 Receptors
Diet
Muscular Diseases
Mast Cells
Wistar Rats
Fasting

Keywords

  • Male rats
  • Metabolic syndrome
  • Muscarinic receptor
  • Purinergic receptor
  • Urinary bladder

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Alterations in peripheral purinergic and muscarinic signaling of rat bladder after long-term fructose-induced metabolic syndrome. / Chung, Shiu Dong; Chien, Chiang Ting; Yu, Hong Jeng.

In: European Journal of Nutrition, Vol. 52, No. 1, 01.02.2013, p. 347-359.

Research output: Contribution to journalArticle

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AB - Purpose: We explored the pathophysiologic mechanisms of long-term fructose-induced lower urinary tract symptoms (LUTS) in rats. Methods: Male Wistar rats were fed with fructose for 3 or 6 months. Biochemical and transcystometric parameters were compared between fructose-fed and age-matched normal-diet rats. Pelvic nerve and external urethral sphincter-electromyogram activity recordings were performed to investigate fructose effects on neural control of bladders. Mitochondrial structure, ATP and acetylcholine content and purinergic and muscarinic cholinergic receptors were examined. Cytosolic cytochrome C staining by Western blot and immunocytochemistry for mitochondrial injury and PGP 9.5 stain for nerve density were also determined. Results: The fructose-fed rats with higher plasma triglyceride, LDL and fasting glucose levels displayed LUTS with increased frequency and suppressed voiding contractile amplitude in phase 1 and phase 2 duration versus normal-diet control. Fructose feeding altered the firing types in pelvic afferent and efferent nerves and external urethral sphincter-electromyogram activity. Increased mast cell number, disrupted and swollen mitochondria, increased cytosolic cytochrome C stain and expression and decreased nerve density in bladder smooth muscle layers appeared in the fructose-fed rats. Fructose feeding also significantly reduced ATP and acetylcholine content and enhanced protein expression of postsynaptic P2X1, P2X 2 and P2X3 purinergic receptors and M 2 and M3 muscarinic cholinergic receptors expression in the smooth muscles of urinary bladder. Conclusion: Long-term fructose feeding induced neuropathy and myopathy in the urinary bladders. Impaired mitochondrial integrity, reduced nerve density, ATP and acetylcholine content and upregulation of purinergic and muscarinic cholinergic receptors expression may contribute to the bladder dysfunction of fructose-fed animals.

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