Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high-fat diet plus streptozotocin-induced diabetic rats

Da Wei Huang; Yangming Martin Lo; Wen Chang Chang; Chia Yu Lin; Jou An Chen; James Swi Bea Wu; Wen Chung Huang; Szu Chuan Shen

doi:10.1002/fsn3.1868

Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high-fat diet plus streptozotocin-induced diabetic rats

Da Wei Huang, Yangming Martin Lo, Wen Chang Chang, Chia Yu Lin, Jou An Chen, James Swi Bea Wu, Wen Chung Huang, Szu Chuan Shen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Ruellia tuberosa L. (RTL) exhibits phytochemical activities and has been used as a folk medicine for curing diabetes mellitus in East Asia for decades. This study investigated the effect of RTL aqueous and ethanolic extracts on nonalcoholic fatty liver disease (NAFLD) and hepatic lipid accumulation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. Administration of RTL aqueous extract (RTLW) or ethanolic extract (RTLE) at dosage of 100 or 400 mg/kg body weight for 4 weeks was carried out in HFD/STZ-induced T2DM rats. Liver weight, adipose (epididymal and perirenal adipose tissues) weight, hepatic triglyceride level, and de novo lipogenesis (DNL)-associated protein expression were monitored after scarification. The results revealed that RTLW and RTLE reduced relative liver weight and relative fat weights in HFD/STZ-induced T2DM rats. RTLW and RTLE also ameliorated NAFLD and hepatic triglyceride (TG) accumulation in diabetic rats. Moreover, hepatic DNL-regulated enzymes such as sterol regulatory element-binding protein-1 (SREBP1) and fatty acid synthase (FAS) expression were significantly suppressed by RTLE (100 and 400 mg/kg body weight) in diabetic rats. The evidences of this study suggest that RTL possesses potential on alleviating NAFLD and lipid accumulation via regulating DNL in the liver of HFD/STZ-induced T2DM rats.

Original language	English
Pages (from-to)	5710-5716
Number of pages	7
Journal	Food Science and Nutrition
Volume	8
Issue number	10
DOIs	https://doi.org/10.1002/fsn3.1868
Publication status	Published - 2020 Oct 1

Keywords

Ruellia tuberosa L.
de novo lipogenesis
hepatic lipid accumulation
type 2 diabetes mellitus

ASJC Scopus subject areas

Food Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/fsn3.1868

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Huang, D. W., Lo, Y. M., Chang, W. C., Lin, C. Y., Chen, J. A., Wu, J. S. B., Huang, W. C., & Shen, S. C. (2020). Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high-fat diet plus streptozotocin-induced diabetic rats. Food Science and Nutrition, 8(10), 5710-5716. https://doi.org/10.1002/fsn3.1868

Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high-fat diet plus streptozotocin-induced diabetic rats. / Huang, Da Wei; Lo, Yangming Martin; Chang, Wen Chang et al.
In: Food Science and Nutrition, Vol. 8, No. 10, 01.10.2020, p. 5710-5716.

Research output: Contribution to journal › Article › peer-review

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title = "Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high-fat diet plus streptozotocin-induced diabetic rats",

abstract = "Ruellia tuberosa L. (RTL) exhibits phytochemical activities and has been used as a folk medicine for curing diabetes mellitus in East Asia for decades. This study investigated the effect of RTL aqueous and ethanolic extracts on nonalcoholic fatty liver disease (NAFLD) and hepatic lipid accumulation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. Administration of RTL aqueous extract (RTLW) or ethanolic extract (RTLE) at dosage of 100 or 400 mg/kg body weight for 4 weeks was carried out in HFD/STZ-induced T2DM rats. Liver weight, adipose (epididymal and perirenal adipose tissues) weight, hepatic triglyceride level, and de novo lipogenesis (DNL)-associated protein expression were monitored after scarification. The results revealed that RTLW and RTLE reduced relative liver weight and relative fat weights in HFD/STZ-induced T2DM rats. RTLW and RTLE also ameliorated NAFLD and hepatic triglyceride (TG) accumulation in diabetic rats. Moreover, hepatic DNL-regulated enzymes such as sterol regulatory element-binding protein-1 (SREBP1) and fatty acid synthase (FAS) expression were significantly suppressed by RTLE (100 and 400 mg/kg body weight) in diabetic rats. The evidences of this study suggest that RTL possesses potential on alleviating NAFLD and lipid accumulation via regulating DNL in the liver of HFD/STZ-induced T2DM rats.",

keywords = "Ruellia tuberosa L., de novo lipogenesis, hepatic lipid accumulation, type 2 diabetes mellitus",

author = "Huang, {Da Wei} and Lo, {Yangming Martin} and Chang, {Wen Chang} and Lin, {Chia Yu} and Chen, {Jou An} and Wu, {James Swi Bea} and Huang, {Wen Chung} and Shen, {Szu Chuan}",

note = "Funding Information: The authors would like to thank the Ministry of Science and Technology of the Republic of China (ROC), Taiwan, for financially supporting this research under contract no. MOST 104-2320-B-003-003-MY3 and no. MOST 107-2320-B-003-004-MY3. This article was also subsidized by the National Taiwan Normal University (NTNU), Taiwan, R.O.C. Funding Information: The authors would like to thank the Ministry of Science and Technology of the Republic of China (ROC), Taiwan, for financially supporting this research under contract no. MOST 104‐2320‐B‐003‐003‐MY3 and no. MOST 107‐2320‐B‐003‐004‐MY3. This article was also subsidized by the National Taiwan Normal University (NTNU), Taiwan, R.O.C. Publisher Copyright: {\textcopyright} 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.",

year = "2020",

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doi = "10.1002/fsn3.1868",

language = "English",

volume = "8",

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T1 - Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high-fat diet plus streptozotocin-induced diabetic rats

AU - Huang, Da Wei

AU - Lo, Yangming Martin

AU - Chang, Wen Chang

AU - Lin, Chia Yu

AU - Chen, Jou An

AU - Wu, James Swi Bea

AU - Huang, Wen Chung

AU - Shen, Szu Chuan

N1 - Funding Information: The authors would like to thank the Ministry of Science and Technology of the Republic of China (ROC), Taiwan, for financially supporting this research under contract no. MOST 104-2320-B-003-003-MY3 and no. MOST 107-2320-B-003-004-MY3. This article was also subsidized by the National Taiwan Normal University (NTNU), Taiwan, R.O.C. Funding Information: The authors would like to thank the Ministry of Science and Technology of the Republic of China (ROC), Taiwan, for financially supporting this research under contract no. MOST 104‐2320‐B‐003‐003‐MY3 and no. MOST 107‐2320‐B‐003‐004‐MY3. This article was also subsidized by the National Taiwan Normal University (NTNU), Taiwan, R.O.C. Publisher Copyright: © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Ruellia tuberosa L. (RTL) exhibits phytochemical activities and has been used as a folk medicine for curing diabetes mellitus in East Asia for decades. This study investigated the effect of RTL aqueous and ethanolic extracts on nonalcoholic fatty liver disease (NAFLD) and hepatic lipid accumulation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. Administration of RTL aqueous extract (RTLW) or ethanolic extract (RTLE) at dosage of 100 or 400 mg/kg body weight for 4 weeks was carried out in HFD/STZ-induced T2DM rats. Liver weight, adipose (epididymal and perirenal adipose tissues) weight, hepatic triglyceride level, and de novo lipogenesis (DNL)-associated protein expression were monitored after scarification. The results revealed that RTLW and RTLE reduced relative liver weight and relative fat weights in HFD/STZ-induced T2DM rats. RTLW and RTLE also ameliorated NAFLD and hepatic triglyceride (TG) accumulation in diabetic rats. Moreover, hepatic DNL-regulated enzymes such as sterol regulatory element-binding protein-1 (SREBP1) and fatty acid synthase (FAS) expression were significantly suppressed by RTLE (100 and 400 mg/kg body weight) in diabetic rats. The evidences of this study suggest that RTL possesses potential on alleviating NAFLD and lipid accumulation via regulating DNL in the liver of HFD/STZ-induced T2DM rats.

AB - Ruellia tuberosa L. (RTL) exhibits phytochemical activities and has been used as a folk medicine for curing diabetes mellitus in East Asia for decades. This study investigated the effect of RTL aqueous and ethanolic extracts on nonalcoholic fatty liver disease (NAFLD) and hepatic lipid accumulation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. Administration of RTL aqueous extract (RTLW) or ethanolic extract (RTLE) at dosage of 100 or 400 mg/kg body weight for 4 weeks was carried out in HFD/STZ-induced T2DM rats. Liver weight, adipose (epididymal and perirenal adipose tissues) weight, hepatic triglyceride level, and de novo lipogenesis (DNL)-associated protein expression were monitored after scarification. The results revealed that RTLW and RTLE reduced relative liver weight and relative fat weights in HFD/STZ-induced T2DM rats. RTLW and RTLE also ameliorated NAFLD and hepatic triglyceride (TG) accumulation in diabetic rats. Moreover, hepatic DNL-regulated enzymes such as sterol regulatory element-binding protein-1 (SREBP1) and fatty acid synthase (FAS) expression were significantly suppressed by RTLE (100 and 400 mg/kg body weight) in diabetic rats. The evidences of this study suggest that RTL possesses potential on alleviating NAFLD and lipid accumulation via regulating DNL in the liver of HFD/STZ-induced T2DM rats.

KW - Ruellia tuberosa L.

KW - de novo lipogenesis

KW - hepatic lipid accumulation

KW - type 2 diabetes mellitus

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Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high-fat diet plus streptozotocin-induced diabetic rats

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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