Acid secretion by mitochondrion-rich cells of medaka (Oryzias latipes) acclimated to acidic freshwater

Chia Cheng Lin, Li Yih Lin, Hao Hsuan Hsu, Violette Thermes, Patrick Prunet, Jiun Lin Horng, Pung Pung Hwang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


In the present study, medaka embryos were exposed to acidified freshwater (pH 5) to investigate the mechanism of acid secretion by mitochondrion-rich (MR) cells in embryonic skin. With double or triple in situ hybridization/ immunocytochemistry, the Na +/H + exchanger 3 (NHE3) and H +-ATPase were localized in two distinct subtypes of MR cells. NHE3 was expressed in apical membranes of a major proportion of MR cells, whereas H +-ATPase was expressed in basolateral membranes of a much smaller proportion of MR cells. Gill mRNA levels of NHE3 and H +-ATPase and the two subtypes of MR cells in yolk sac skin were increased by acid acclimation; however, the mRNA level of NHE3 was remarkably higher than that of H +-ATPase. A scanning ion-selective electrode technique was used to measure H +, Na +, and NH4 + transport by individual MR cells in larval skin. Results showed that Na + uptake and NH4 + excretion by MR cells increased after acid acclimation. These findings suggested that the NHE3/Rh glycoprotein-mediated Na + uptake/NH4 + excretion mechanism plays a critical role in acidic equivalent (H +/NH4 +) excretion by MR cells of the freshwater medaka.

Original languageEnglish
Pages (from-to)R283-R291
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Issue number2
Publication statusPublished - 2012 Jan


  • Ionocytes
  • Na /H exchanger
  • Rh glycoprotein

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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