Absence of radius and ulna in mice lacking hoxa-11 andhoxd-11

Allan Peter Davis, David P. Witte, Hsiu M. Hsieh-Li, S. Steven Potter, Mario R. Capecchi

Research output: Contribution to journalArticle

464 Citations (Scopus)

Abstract

MICE with targeted disruptions1 in Hoxgenes have been generated to evaluate the role of the Hox complex in determining the mammalian body plan. This complex of 38 genes encodes transcription factors that specify regional information along the embryonic axes. Early in vertebrate evolution an ancestral complex shared with invertebrates was duplicated twice to give rise to the four linkage groups (Hox A, B, C and D)2, 3. As a consequence, corresponding genes on the separate linkage groups, called paralogues, are most closely related to each other. Based on sequence similarities, the Hox genes have been subdivided into 13 paralogous groups. The five most 5′ groups (Hox9-13) pattern the posterior region of the vertebrate embryo and the appendicular skeleton4-18. Mice with individual mutations in the paralogous genes hoxa-11 and hoxd-11 have been described15-18. By breeding these two strains together we have generated double mutants which have dramatic phenotypes not apparent in mice homozygous for the individual mutations. The radius and the ulna of the forelimb are almost entirely eliminated, the axial skeleton shows homeotic transformations, and there are severe kidney defects not present in either single mutant. The limb and axial phenotypes are quantitative: As more mutant alleles are added to the genotype, the phenotype becomes progressively more severe. The appendicular skeleton defects suggest that paralogous Hoxgenes function together to specify limb outgrowth and patterning along the proximodistal axis.

Original languageEnglish
Pages (from-to)791-795
Number of pages5
JournalNature
Volume375
Issue number6534
DOIs
Publication statusPublished - 1995 Jun

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Ulna
Phenotype
Skeleton
Vertebrates
Extremities
Genes
Mutation
Forelimb
Homeobox Genes
Invertebrates
Breeding
Transcription Factors
Embryonic Structures
Alleles
Genotype
Kidney

ASJC Scopus subject areas

  • General

Cite this

Davis, A. P., Witte, D. P., Hsieh-Li, H. M., Potter, S. S., & Capecchi, M. R. (1995). Absence of radius and ulna in mice lacking hoxa-11 andhoxd-11. Nature, 375(6534), 791-795. https://doi.org/10.1038/375791a0

Absence of radius and ulna in mice lacking hoxa-11 andhoxd-11. / Davis, Allan Peter; Witte, David P.; Hsieh-Li, Hsiu M.; Potter, S. Steven; Capecchi, Mario R.

In: Nature, Vol. 375, No. 6534, 06.1995, p. 791-795.

Research output: Contribution to journalArticle

Davis, AP, Witte, DP, Hsieh-Li, HM, Potter, SS & Capecchi, MR 1995, 'Absence of radius and ulna in mice lacking hoxa-11 andhoxd-11', Nature, vol. 375, no. 6534, pp. 791-795. https://doi.org/10.1038/375791a0
Davis AP, Witte DP, Hsieh-Li HM, Potter SS, Capecchi MR. Absence of radius and ulna in mice lacking hoxa-11 andhoxd-11. Nature. 1995 Jun;375(6534):791-795. https://doi.org/10.1038/375791a0
Davis, Allan Peter ; Witte, David P. ; Hsieh-Li, Hsiu M. ; Potter, S. Steven ; Capecchi, Mario R. / Absence of radius and ulna in mice lacking hoxa-11 andhoxd-11. In: Nature. 1995 ; Vol. 375, No. 6534. pp. 791-795.
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