Abnormal uterine stromal and glandular function associated with maternal reproductive defects in Hoxa-11 null mice

Robert L. Gendron, Hélène Paradis, Hsiu M. Hsieh-Li, David W. Lee, S. Steven Potter, Edith Markoff

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Here we describe in detail both the expression of Hoxa-11 in the wild- type mouse uterus and the defects resulting in maternal reproductive failure of Hoxa-11 null female mice. The Hoxa-11 gene is expressed at peak levels in uterine stromal cells during metestrus. Hoxa-11 transcripts were induced beginning on Day 2 of gestation in the stromal cells underlying the uterine epithelium and appeared in the secondary decidual zone between Days 6 and 8 of gestation. At early gestational stages, stromal, decidual, and glandular cell development were deficient in Hoxa-11 null uteri in comparison to wild- type as assessed by histology and immunohistochemical localization of the decidual cell marker epitope, stage-specific embryonic antigen-3 (SSEA-3). Both steroid-induced uterine stromal and glandular cell proliferation as well as oil-induced stromal decidualization after induction of pseudopregnancy were deficient in mutant uteri. Moreover, both Western blotting and immunohistochemistry demonstrated that the burst of glandular leukemia inhibitory factor (LIF) found in normal pregnant uteri at Day 4.5 of gestation was absent in Hoxa-11-deficient uteri. The LIF burst was also not observed in the uteri of bilaterally ovariectomized, hormonally stimulated Hoxa-11 mutants. These results demonstrate that the Hoxa-11 gene is required for normal uterine stromal cell and glandular differentiation during pregnancy, as is the presence of the steroid-induced glandular LIF burst initiating embryo implantation.

Original languageEnglish
Pages (from-to)1097-1105
Number of pages9
JournalBiology of Reproduction
Volume56
Issue number5
DOIs
Publication statusPublished - 1997 May

Fingerprint

Uterus
Mothers
Stromal Cells
Leukemia Inhibitory Factor
Pregnancy
Steroids
Metestrus
Pseudopregnancy
Oil and Gas Fields
Genes
Epitopes
Cell Differentiation
Histology
Epithelium
Western Blotting
Immunohistochemistry
Cell Proliferation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Abnormal uterine stromal and glandular function associated with maternal reproductive defects in Hoxa-11 null mice. / Gendron, Robert L.; Paradis, Hélène; Hsieh-Li, Hsiu M.; Lee, David W.; Potter, S. Steven; Markoff, Edith.

In: Biology of Reproduction, Vol. 56, No. 5, 05.1997, p. 1097-1105.

Research output: Contribution to journalArticle

Gendron, Robert L. ; Paradis, Hélène ; Hsieh-Li, Hsiu M. ; Lee, David W. ; Potter, S. Steven ; Markoff, Edith. / Abnormal uterine stromal and glandular function associated with maternal reproductive defects in Hoxa-11 null mice. In: Biology of Reproduction. 1997 ; Vol. 56, No. 5. pp. 1097-1105.
@article{50be121fa5af4625a69c6248ac7217ed,
title = "Abnormal uterine stromal and glandular function associated with maternal reproductive defects in Hoxa-11 null mice",
abstract = "Here we describe in detail both the expression of Hoxa-11 in the wild- type mouse uterus and the defects resulting in maternal reproductive failure of Hoxa-11 null female mice. The Hoxa-11 gene is expressed at peak levels in uterine stromal cells during metestrus. Hoxa-11 transcripts were induced beginning on Day 2 of gestation in the stromal cells underlying the uterine epithelium and appeared in the secondary decidual zone between Days 6 and 8 of gestation. At early gestational stages, stromal, decidual, and glandular cell development were deficient in Hoxa-11 null uteri in comparison to wild- type as assessed by histology and immunohistochemical localization of the decidual cell marker epitope, stage-specific embryonic antigen-3 (SSEA-3). Both steroid-induced uterine stromal and glandular cell proliferation as well as oil-induced stromal decidualization after induction of pseudopregnancy were deficient in mutant uteri. Moreover, both Western blotting and immunohistochemistry demonstrated that the burst of glandular leukemia inhibitory factor (LIF) found in normal pregnant uteri at Day 4.5 of gestation was absent in Hoxa-11-deficient uteri. The LIF burst was also not observed in the uteri of bilaterally ovariectomized, hormonally stimulated Hoxa-11 mutants. These results demonstrate that the Hoxa-11 gene is required for normal uterine stromal cell and glandular differentiation during pregnancy, as is the presence of the steroid-induced glandular LIF burst initiating embryo implantation.",
author = "Gendron, {Robert L.} and H{\'e}l{\`e}ne Paradis and Hsieh-Li, {Hsiu M.} and Lee, {David W.} and Potter, {S. Steven} and Edith Markoff",
year = "1997",
month = "5",
doi = "10.1095/biolreprod56.5.1097",
language = "English",
volume = "56",
pages = "1097--1105",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "5",

}

TY - JOUR

T1 - Abnormal uterine stromal and glandular function associated with maternal reproductive defects in Hoxa-11 null mice

AU - Gendron, Robert L.

AU - Paradis, Hélène

AU - Hsieh-Li, Hsiu M.

AU - Lee, David W.

AU - Potter, S. Steven

AU - Markoff, Edith

PY - 1997/5

Y1 - 1997/5

N2 - Here we describe in detail both the expression of Hoxa-11 in the wild- type mouse uterus and the defects resulting in maternal reproductive failure of Hoxa-11 null female mice. The Hoxa-11 gene is expressed at peak levels in uterine stromal cells during metestrus. Hoxa-11 transcripts were induced beginning on Day 2 of gestation in the stromal cells underlying the uterine epithelium and appeared in the secondary decidual zone between Days 6 and 8 of gestation. At early gestational stages, stromal, decidual, and glandular cell development were deficient in Hoxa-11 null uteri in comparison to wild- type as assessed by histology and immunohistochemical localization of the decidual cell marker epitope, stage-specific embryonic antigen-3 (SSEA-3). Both steroid-induced uterine stromal and glandular cell proliferation as well as oil-induced stromal decidualization after induction of pseudopregnancy were deficient in mutant uteri. Moreover, both Western blotting and immunohistochemistry demonstrated that the burst of glandular leukemia inhibitory factor (LIF) found in normal pregnant uteri at Day 4.5 of gestation was absent in Hoxa-11-deficient uteri. The LIF burst was also not observed in the uteri of bilaterally ovariectomized, hormonally stimulated Hoxa-11 mutants. These results demonstrate that the Hoxa-11 gene is required for normal uterine stromal cell and glandular differentiation during pregnancy, as is the presence of the steroid-induced glandular LIF burst initiating embryo implantation.

AB - Here we describe in detail both the expression of Hoxa-11 in the wild- type mouse uterus and the defects resulting in maternal reproductive failure of Hoxa-11 null female mice. The Hoxa-11 gene is expressed at peak levels in uterine stromal cells during metestrus. Hoxa-11 transcripts were induced beginning on Day 2 of gestation in the stromal cells underlying the uterine epithelium and appeared in the secondary decidual zone between Days 6 and 8 of gestation. At early gestational stages, stromal, decidual, and glandular cell development were deficient in Hoxa-11 null uteri in comparison to wild- type as assessed by histology and immunohistochemical localization of the decidual cell marker epitope, stage-specific embryonic antigen-3 (SSEA-3). Both steroid-induced uterine stromal and glandular cell proliferation as well as oil-induced stromal decidualization after induction of pseudopregnancy were deficient in mutant uteri. Moreover, both Western blotting and immunohistochemistry demonstrated that the burst of glandular leukemia inhibitory factor (LIF) found in normal pregnant uteri at Day 4.5 of gestation was absent in Hoxa-11-deficient uteri. The LIF burst was also not observed in the uteri of bilaterally ovariectomized, hormonally stimulated Hoxa-11 mutants. These results demonstrate that the Hoxa-11 gene is required for normal uterine stromal cell and glandular differentiation during pregnancy, as is the presence of the steroid-induced glandular LIF burst initiating embryo implantation.

UR - http://www.scopus.com/inward/record.url?scp=0030998546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030998546&partnerID=8YFLogxK

U2 - 10.1095/biolreprod56.5.1097

DO - 10.1095/biolreprod56.5.1097

M3 - Article

C2 - 9160706

AN - SCOPUS:0030998546

VL - 56

SP - 1097

EP - 1105

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 5

ER -