A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

Li Xing Yang; Yu Cheng Liu; Chang Hui Cho; Yi Rou Chen; Chan Shan Yang; Yin Lin Lu; Zhiming Zhang; Yi Tseng Tsai; Yu Cheng Chin; Jiashing Yu; Hsiu Min Pan; Wei Rou Jiang; Zi Chun Chia; Wei Shiang Huang; Yu Lin Chiu; Chun Kai Sun; Yu Ting Huang; Li Ming Chen; Ken Tsung Wong; Han Min Huang; Chih Hsin Chen; Yuan Jay Chang; Chih Chia Huang; Tzu Ming Liu

doi:10.1186/s12951-022-01515-5

A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

Li Xing Yang, Yu Cheng Liu, Chang Hui Cho, Yi Rou Chen, Chan Shan Yang, Yin Lin Lu, Zhiming Zhang, Yi Tseng Tsai, Yu Cheng Chin, Jiashing Yu, Hsiu Min Pan, Wei Rou Jiang, Zi Chun Chia, Wei Shiang Huang, Yu Lin Chiu, Chun Kai Sun, Yu Ting Huang, Li Ming Chen, Ken Tsung Wong, Han Min HuangChih Hsin Chen^*, Yuan Jay Chang^*, Chih Chia Huang^*, Tzu Ming Liu^*

^*Corresponding author for this work

Graduate Institute of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic dyes are water-insoluble such that the derivatization and modification of these dyes are difficult. Herein, we demonstrated a simple platform for the fabrication of organic NPs designed with emissive properties by loading ten different organic dyes (molar masses of 479.1–1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). The result showed a substantial improvement over the loading of commercial dyes (3.7–50% loading) while preventing their spontaneous aggregation in aqueous solutions. This packaging strategy includes our newly synthesized organic dyes (> 85% loading) designed for OPVs (242), DSSCs (YI-1, YI-3, YI-8), and OLEDs (ADF-1–3, and DTDPTID) applications. These low-cytotoxicity organic NPs exhibited tunable fluorescence from visible to near-infrared (NIR) emission for cellular imaging and biological tracking in vivo. Moreover, PSMA NPs loaded with designed NIR-dyes were fabricated, and photodynamic therapy with these dye-loaded PSMA NPs for the photolysis of cancer cells was achieved when coupled with 808 nm laser excitation. Indeed, our work demonstrates a facile approach for increasing the biocompatibility and stability of organic dyes by loading them into water-soluble polymer-based carriers, providing a new perspective of organic optoelectronic materials in biomedical theranostic applications.

Original language	English
Article number	311
Journal	Journal of Nanobiotechnology
Volume	20
Issue number	1
DOIs	https://doi.org/10.1186/s12951-022-01515-5
Publication status	Published - 2022 Dec

Keywords

Fluorescence imaging
Multiphoton microscopy
Near-infrared red
Optoelectronic material
Photodynamic therapy
Polymer
Theranostic

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1186/s12951-022-01515-5

Cite this

Yang, L. X., Liu, Y. C., Cho, C. H., Chen, Y. R., Yang, C. S., Lu, Y. L., Zhang, Z., Tsai, Y. T., Chin, Y. C., Yu, J., Pan, H. M., Jiang, W. R., Chia, Z. C., Huang, W. S., Chiu, Y. L., Sun, C. K., Huang, Y. T., Chen, L. M., Wong, K. T., ... Liu, T. M. (2022). A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges. Journal of Nanobiotechnology, 20(1), Article 311. https://doi.org/10.1186/s12951-022-01515-5

Yang, LX, Liu, YC, Cho, CH, Chen, YR, Yang, CS, Lu, YL, Zhang, Z, Tsai, YT, Chin, YC, Yu, J, Pan, HM, Jiang, WR, Chia, ZC, Huang, WS, Chiu, YL, Sun, CK, Huang, YT, Chen, LM, Wong, KT, Huang, HM, Chen, CH, Chang, YJ, Huang, CC & Liu, TM 2022, 'A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges', Journal of Nanobiotechnology, vol. 20, no. 1, 311. https://doi.org/10.1186/s12951-022-01515-5

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title = "A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges",

abstract = "The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic dyes are water-insoluble such that the derivatization and modification of these dyes are difficult. Herein, we demonstrated a simple platform for the fabrication of organic NPs designed with emissive properties by loading ten different organic dyes (molar masses of 479.1–1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). The result showed a substantial improvement over the loading of commercial dyes (3.7–50% loading) while preventing their spontaneous aggregation in aqueous solutions. This packaging strategy includes our newly synthesized organic dyes (> 85% loading) designed for OPVs (242), DSSCs (YI-1, YI-3, YI-8), and OLEDs (ADF-1–3, and DTDPTID) applications. These low-cytotoxicity organic NPs exhibited tunable fluorescence from visible to near-infrared (NIR) emission for cellular imaging and biological tracking in vivo. Moreover, PSMA NPs loaded with designed NIR-dyes were fabricated, and photodynamic therapy with these dye-loaded PSMA NPs for the photolysis of cancer cells was achieved when coupled with 808 nm laser excitation. Indeed, our work demonstrates a facile approach for increasing the biocompatibility and stability of organic dyes by loading them into water-soluble polymer-based carriers, providing a new perspective of organic optoelectronic materials in biomedical theranostic applications.",

keywords = "Fluorescence imaging, Multiphoton microscopy, Near-infrared red, Optoelectronic material, Photodynamic therapy, Polymer, Theranostic",

author = "Yang, {Li Xing} and Liu, {Yu Cheng} and Cho, {Chang Hui} and Chen, {Yi Rou} and Yang, {Chan Shan} and Lu, {Yin Lin} and Zhiming Zhang and Tsai, {Yi Tseng} and Chin, {Yu Cheng} and Jiashing Yu and Pan, {Hsiu Min} and Jiang, {Wei Rou} and Chia, {Zi Chun} and Huang, {Wei Shiang} and Chiu, {Yu Lin} and Sun, {Chun Kai} and Huang, {Yu Ting} and Chen, {Li Ming} and Wong, {Ken Tsung} and Huang, {Han Min} and Chen, {Chih Hsin} and Chang, {Yuan Jay} and Huang, {Chih Chia} and Liu, {Tzu Ming}",

note = "Funding Information: This work was sponsored by the Faculty of Health Sciences, University of Macau, the internal funding of the University of Macau under MYRG2018-00070-FHS, the grants funded by The Science and Technology Development Fund, Macau SAR (File No. 122/2016/A3, 018/2017/A1, FDCT/0011/2019/AKP, 0120/2020/A3 and 0026/2021/A), the Ministry of Science and Technology, Taiwan (MOST 108-2113-M-006-012-MY3, MOST 109-2113-M-029-009, MOST 109-2327-B-009-001, MOST 109-2113-M-032-002, MOST 108-2314-B-039-007-MY3, MOST 107-2113-M-002-019-MY3, MOST 110-2731-M-006-001, MOST 110-2221-E-002-013, and MOST 110-2112-M-003-012-MY3), the Center of Applied Nanomedicine, National Cheng Kung University from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan. The authors gratefully acknowledge the use of EM000800 belonging to the Core Facility Center of National Cheng Kung University. Funding Information: This work was sponsored by the Faculty of Health Sciences, University of Macau, the internal funding of the University of Macau under MYRG2018-00070-FHS, the Grants funded by The Science and Technology Development Fund, Macau SAR (File no. 122/2016/A3, 018/2017/A1, FDCT/0011/2019/AKP, 0120/2020/A3 and 0026/2021/A), the Ministry of Science and Technology, Taiwan (MOST 108-2113-M-006-012-MY3, MOST 109-2113-M-029-009, MOST 109-2327-B-009-001, MOST 109-2113-M-032-002, MOST 108-2314-B-039-007-MY3, MOST 107-2113-M-002-019-MY3, MOST 110-2731-M-006-001, MOST 110-2221-E-002-013, and MOST 110-2112-M-003-012-MY3). Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1186/s12951-022-01515-5",

language = "English",

volume = "20",

journal = "Journal of Nanobiotechnology",

issn = "1477-3155",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

AU - Yang, Li Xing

AU - Liu, Yu Cheng

AU - Cho, Chang Hui

AU - Chen, Yi Rou

AU - Yang, Chan Shan

AU - Lu, Yin Lin

AU - Zhang, Zhiming

AU - Tsai, Yi Tseng

AU - Chin, Yu Cheng

AU - Yu, Jiashing

AU - Pan, Hsiu Min

AU - Jiang, Wei Rou

AU - Chia, Zi Chun

AU - Huang, Wei Shiang

AU - Chiu, Yu Lin

AU - Sun, Chun Kai

AU - Huang, Yu Ting

AU - Chen, Li Ming

AU - Wong, Ken Tsung

AU - Huang, Han Min

AU - Chen, Chih Hsin

AU - Chang, Yuan Jay

AU - Huang, Chih Chia

AU - Liu, Tzu Ming

N1 - Funding Information: This work was sponsored by the Faculty of Health Sciences, University of Macau, the internal funding of the University of Macau under MYRG2018-00070-FHS, the grants funded by The Science and Technology Development Fund, Macau SAR (File No. 122/2016/A3, 018/2017/A1, FDCT/0011/2019/AKP, 0120/2020/A3 and 0026/2021/A), the Ministry of Science and Technology, Taiwan (MOST 108-2113-M-006-012-MY3, MOST 109-2113-M-029-009, MOST 109-2327-B-009-001, MOST 109-2113-M-032-002, MOST 108-2314-B-039-007-MY3, MOST 107-2113-M-002-019-MY3, MOST 110-2731-M-006-001, MOST 110-2221-E-002-013, and MOST 110-2112-M-003-012-MY3), the Center of Applied Nanomedicine, National Cheng Kung University from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan. The authors gratefully acknowledge the use of EM000800 belonging to the Core Facility Center of National Cheng Kung University. Funding Information: This work was sponsored by the Faculty of Health Sciences, University of Macau, the internal funding of the University of Macau under MYRG2018-00070-FHS, the Grants funded by The Science and Technology Development Fund, Macau SAR (File no. 122/2016/A3, 018/2017/A1, FDCT/0011/2019/AKP, 0120/2020/A3 and 0026/2021/A), the Ministry of Science and Technology, Taiwan (MOST 108-2113-M-006-012-MY3, MOST 109-2113-M-029-009, MOST 109-2327-B-009-001, MOST 109-2113-M-032-002, MOST 108-2314-B-039-007-MY3, MOST 107-2113-M-002-019-MY3, MOST 110-2731-M-006-001, MOST 110-2221-E-002-013, and MOST 110-2112-M-003-012-MY3). Publisher Copyright: © 2022, The Author(s).

PY - 2022/12

Y1 - 2022/12

N2 - The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic dyes are water-insoluble such that the derivatization and modification of these dyes are difficult. Herein, we demonstrated a simple platform for the fabrication of organic NPs designed with emissive properties by loading ten different organic dyes (molar masses of 479.1–1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). The result showed a substantial improvement over the loading of commercial dyes (3.7–50% loading) while preventing their spontaneous aggregation in aqueous solutions. This packaging strategy includes our newly synthesized organic dyes (> 85% loading) designed for OPVs (242), DSSCs (YI-1, YI-3, YI-8), and OLEDs (ADF-1–3, and DTDPTID) applications. These low-cytotoxicity organic NPs exhibited tunable fluorescence from visible to near-infrared (NIR) emission for cellular imaging and biological tracking in vivo. Moreover, PSMA NPs loaded with designed NIR-dyes were fabricated, and photodynamic therapy with these dye-loaded PSMA NPs for the photolysis of cancer cells was achieved when coupled with 808 nm laser excitation. Indeed, our work demonstrates a facile approach for increasing the biocompatibility and stability of organic dyes by loading them into water-soluble polymer-based carriers, providing a new perspective of organic optoelectronic materials in biomedical theranostic applications.

AB - The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic dyes are water-insoluble such that the derivatization and modification of these dyes are difficult. Herein, we demonstrated a simple platform for the fabrication of organic NPs designed with emissive properties by loading ten different organic dyes (molar masses of 479.1–1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). The result showed a substantial improvement over the loading of commercial dyes (3.7–50% loading) while preventing their spontaneous aggregation in aqueous solutions. This packaging strategy includes our newly synthesized organic dyes (> 85% loading) designed for OPVs (242), DSSCs (YI-1, YI-3, YI-8), and OLEDs (ADF-1–3, and DTDPTID) applications. These low-cytotoxicity organic NPs exhibited tunable fluorescence from visible to near-infrared (NIR) emission for cellular imaging and biological tracking in vivo. Moreover, PSMA NPs loaded with designed NIR-dyes were fabricated, and photodynamic therapy with these dye-loaded PSMA NPs for the photolysis of cancer cells was achieved when coupled with 808 nm laser excitation. Indeed, our work demonstrates a facile approach for increasing the biocompatibility and stability of organic dyes by loading them into water-soluble polymer-based carriers, providing a new perspective of organic optoelectronic materials in biomedical theranostic applications.

KW - Fluorescence imaging

KW - Multiphoton microscopy

KW - Near-infrared red

KW - Optoelectronic material

KW - Photodynamic therapy

KW - Polymer

KW - Theranostic

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U2 - 10.1186/s12951-022-01515-5

DO - 10.1186/s12951-022-01515-5

M3 - Article

C2 - 35794602

AN - SCOPUS:85133547002

SN - 1477-3155

VL - 20

JO - Journal of Nanobiotechnology

JF - Journal of Nanobiotechnology

IS - 1

M1 - 311

ER -

A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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