A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes

G. Nana Hagan, Yenshou Lin, Mark A. Magnuson, Joseph Avruch, Michael P. Czech

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Insulin signaling through phosphatidylinositol 3-kinase (PI 3-kinase) activates the protein kinase Akt through phosphorylation of its threonine 308 and serine 473 residues by the PDK1 protein kinase and the Rictor-mammalian target of rapamycin complex (mTORC2), respectively. Remarkably, we show here that the Rictor protein is also present in cultured adipocytes in complexes containing Myo1c, a molecular motor that promotes cortical actin remodeling. Interestingly, the Rictor-Myo1c complex is biochemically distinct from the previously reported mTORC2 and can be immunoprecipitated independently of mTORC2. Furthermore, while RNA interference-directed silencing of Rictor results in the expected attenuation of Akt phosphorylation at serine 473, depletion of Myo1c is without effect. In contrast, loss of either Rictor or Myo1c inhibits phosphorylation of the actin filament regulatory protein paxillin at tyrosine 118. Furthermore, Myo1c-induced membrane ruffling of 3T3-L1 adipocytes is also compromised following Rictor knockdown. Interestingly, neither the mTORC2 inhibitor rapamycin nor the PI 3-kinase inhibitor wortmannin affects paxillin tyrosine 118 phosphorylation. Taken together, our findings suggest that the Rictor-Myo1c complex is distinct from mTORC2 and that Myo1c, in conjunction with Rictor, participates in cortical actin remodeling events.

Original languageEnglish
Pages (from-to)4215-4226
Number of pages12
JournalMolecular and cellular biology
Volume28
Issue number13
DOIs
Publication statusPublished - 2008 Jul 1

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Adipocytes
Actins
Phosphorylation
Phosphatidylinositol 3-Kinase
Paxillin
Sirolimus
Protein Kinases
Serine
Tyrosine
Threonine
RNA Interference
Actin Cytoskeleton
Proteins
TOR complex 2
Insulin
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes. / Hagan, G. Nana; Lin, Yenshou; Magnuson, Mark A.; Avruch, Joseph; Czech, Michael P.

In: Molecular and cellular biology, Vol. 28, No. 13, 01.07.2008, p. 4215-4226.

Research output: Contribution to journalArticle

Hagan, G. Nana ; Lin, Yenshou ; Magnuson, Mark A. ; Avruch, Joseph ; Czech, Michael P. / A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes. In: Molecular and cellular biology. 2008 ; Vol. 28, No. 13. pp. 4215-4226.
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