A RFID tag based remote DNA sensing system

J. H. Chien; C. Y. Tsai; Y. T. Cheng; C. R. Yang; P. Y. Wang; T. L. Chang; D. S. Lee; C. W. Chang; W. P. Chou; C. C. Chiang; Y. W. Lee; P. H. Chen

doi:10.1109/NEMS.2006.334722

A RFID tag based remote DNA sensing system

J. H. Chien, C. Y. Tsai, Y. T. Cheng, C. R. Yang, P. Y. Wang, T. L. Chang, D. S. Lee, C. W. Chang, W. P. Chou, C. C. Chiang, Y. W. Lee, P. H. Chen

Department of Mechatronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP ) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the micro capacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radio frequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection.

Original language	English
Title of host publication	Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
Pages	278-282
Number of pages	5
DOIs	https://doi.org/10.1109/NEMS.2006.334722
Publication status	Published - 2006
Event	1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS - Zhuhai, China Duration: 2006 Jan 18 → 2006 Jan 21

Publication series

Name	Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

Other

Other	1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
Country/Territory	China
City	Zhuhai
Period	2006/01/18 → 2006/01/21

Keywords

Diagnostic detection
Gold nanoparticles
RFID
Self-assembly

ASJC Scopus subject areas

Biotechnology
Materials Science (miscellaneous)

Access to Document

10.1109/NEMS.2006.334722

Cite this

Chien, J. H., Tsai, C. Y., Cheng, Y. T., Yang, C. R., Wang, P. Y., Chang, T. L., Lee, D. S., Chang, C. W., Chou, W. P., Chiang, C. C., Lee, Y. W., & Chen, P. H. (2006). A RFID tag based remote DNA sensing system. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS (pp. 278-282). Article 4134952 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334722

A RFID tag based remote DNA sensing system. / Chien, J. H.; Tsai, C. Y.; Cheng, Y. T. et al.
Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 278-282 4134952 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chien, JH, Tsai, CY, Cheng, YT, Yang, CR, Wang, PY, Chang, TL, Lee, DS, Chang, CW, Chou, WP, Chiang, CC, Lee, YW & Chen, PH 2006, A RFID tag based remote DNA sensing system. in Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS., 4134952, Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, pp. 278-282, 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, Zhuhai, China, 2006/01/18. https://doi.org/10.1109/NEMS.2006.334722

Chien JH, Tsai CY, Cheng YT, Yang CR, Wang PY, Chang TL et al. A RFID tag based remote DNA sensing system. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 278-282. 4134952. (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). doi: 10.1109/NEMS.2006.334722

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abstract = "Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP ) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the micro capacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radio frequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection.",

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AU - Lee, D. S.

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AB - Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP ) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the micro capacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radio frequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection.

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A RFID tag based remote DNA sensing system

Abstract

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Keywords

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