A RFID tag based remote DNA sensing system

J. H. Chien, C. Y. Tsai, Y. T. Cheng, C. R. Yang, P. Y. Wang, T. L. Chang, D. S. Lee, C. W. Chang, W. P. Chou, C. C. Chiang, Y. W. Lee, P. H. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP ) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the micro capacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radio frequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection.

Original languageEnglish
Title of host publicationProceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
Pages278-282
Number of pages5
DOIs
Publication statusPublished - 2006 Dec 1
Event1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS - Zhuhai, China
Duration: 2006 Jan 182006 Jan 21

Publication series

NameProceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

Other

Other1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
CountryChina
CityZhuhai
Period06/1/1806/1/21

Keywords

  • Diagnostic detection
  • Gold nanoparticles
  • RFID
  • Self-assembly

ASJC Scopus subject areas

  • Biotechnology
  • Materials Science (miscellaneous)

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  • Cite this

    Chien, J. H., Tsai, C. Y., Cheng, Y. T., Yang, C. R., Wang, P. Y., Chang, T. L., Lee, D. S., Chang, C. W., Chou, W. P., Chiang, C. C., Lee, Y. W., & Chen, P. H. (2006). A RFID tag based remote DNA sensing system. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS (pp. 278-282). [4134952] (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334722