A reversible surface functionalized nanowire transistor to study protein-protein interactions

Shu Ping Lin, Chien Yuan Pan, Kun Chang Tseng, Ming Chou Lin, Chii Dong Chen, Chia Chang Tsai, Shu Han Yu, Ying Chieh Sun, Tsung Wu Lin, Yit Tsong Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)


Taking advantage of the reversible association-dissociation between glutathione (GSH) and glutathione S-transferase (GST), we have developed a novel method by surface-functionalizing GSH on silicon nanowire field-effect transistors (SiNW-FET) and then anchoring a particular GST-fused protein for the studies of protein-protein interactions. The reversible surface functionalization method has been proven by analytical techniques, fluorescence imaging, atomic force microscopic imaging, and electrical measurements. The reversible GSH-GST functionality on the SiNW-FET has made this sensorial device consecutively reusable and calibratable, allowing for quantitative analysis. In this work, we have demonstrated that the biologically modified SiNW-FET can be used as a screening sensor for the future studies of biomolecular associations, such as protein-protein interactions, protein-DNA interactions, protein-carbohydrate interactions, etc.

Original languageEnglish
Pages (from-to)235-243
Number of pages9
JournalNano Today
Issue number3
Publication statusPublished - 2009 Jun


  • Field-effect transistor
  • Nanowire
  • Protein-protein interaction
  • Reversible surface functionalization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • General Materials Science
  • Pharmaceutical Science


Dive into the research topics of 'A reversible surface functionalized nanowire transistor to study protein-protein interactions'. Together they form a unique fingerprint.

Cite this