A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions

Yi Ju Chen; Cheng You Liu; Dong Yu Tsai; Yi Chun Yeh

doi:10.1016/j.snb.2017.12.056

A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions

Yi Ju Chen, Cheng You Liu, Dong Yu Tsai, Yi Chun Yeh^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

In this study, we employed CupR protein in the construct of a fluorescence resonance energy transfer (FRET)-based protein biosensor for the detection of silver ions. We fused the CFP and the YPet through a truncated CupR protein (containing a dimerization helix and a metal binding domain) to create a FRET-based biosensor for the detection of silver ions. Various aqueous media (including tap water and pond water) were examined to determine the matrix effects and agarose gel was used to increase the feasibility of this portable biosensors. The resulting biosensor has demonstrated high sensitivity and selectivity to silver ions.

Original language	English
Pages (from-to)	784-788
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	259
DOIs	https://doi.org/10.1016/j.snb.2017.12.056
Publication status	Published - 2018 Apr 15

Keywords

CupR
FRET
Fluorescence resonance energy transfer
Silver biosensor

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2017.12.056

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title = "A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions",

abstract = "In this study, we employed CupR protein in the construct of a fluorescence resonance energy transfer (FRET)-based protein biosensor for the detection of silver ions. We fused the CFP and the YPet through a truncated CupR protein (containing a dimerization helix and a metal binding domain) to create a FRET-based biosensor for the detection of silver ions. Various aqueous media (including tap water and pond water) were examined to determine the matrix effects and agarose gel was used to increase the feasibility of this portable biosensors. The resulting biosensor has demonstrated high sensitivity and selectivity to silver ions.",

keywords = "CupR, FRET, Fluorescence resonance energy transfer, Silver biosensor",

author = "Chen, {Yi Ju} and Liu, {Cheng You} and Tsai, {Dong Yu} and Yeh, {Yi Chun}",

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year = "2018",

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T1 - A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions

AU - Chen, Yi Ju

AU - Liu, Cheng You

AU - Tsai, Dong Yu

AU - Yeh, Yi Chun

PY - 2018/4/15

Y1 - 2018/4/15

N2 - In this study, we employed CupR protein in the construct of a fluorescence resonance energy transfer (FRET)-based protein biosensor for the detection of silver ions. We fused the CFP and the YPet through a truncated CupR protein (containing a dimerization helix and a metal binding domain) to create a FRET-based biosensor for the detection of silver ions. Various aqueous media (including tap water and pond water) were examined to determine the matrix effects and agarose gel was used to increase the feasibility of this portable biosensors. The resulting biosensor has demonstrated high sensitivity and selectivity to silver ions.

AB - In this study, we employed CupR protein in the construct of a fluorescence resonance energy transfer (FRET)-based protein biosensor for the detection of silver ions. We fused the CFP and the YPet through a truncated CupR protein (containing a dimerization helix and a metal binding domain) to create a FRET-based biosensor for the detection of silver ions. Various aqueous media (including tap water and pond water) were examined to determine the matrix effects and agarose gel was used to increase the feasibility of this portable biosensors. The resulting biosensor has demonstrated high sensitivity and selectivity to silver ions.

KW - CupR

KW - FRET

KW - Fluorescence resonance energy transfer

KW - Silver biosensor

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M3 - Article

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SN - 0925-4005

VL - 259

SP - 784

EP - 788

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions

Abstract

Keywords

ASJC Scopus subject areas

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