A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions

Yi Ju Chen, Cheng You Liu, Dong Yu Tsai, Yi Chun Yeh

Research output: Contribution to journalArticle

Abstract

In this study, we employed CupR protein in the construct of a fluorescence resonance energy transfer (FRET)-based protein biosensor for the detection of silver ions. We fused the CFP and the YPet through a truncated CupR protein (containing a dimerization helix and a metal binding domain) to create a FRET-based biosensor for the detection of silver ions. Various aqueous media (including tap water and pond water) were examined to determine the matrix effects and agarose gel was used to increase the feasibility of this portable biosensors. The resulting biosensor has demonstrated high sensitivity and selectivity to silver ions.

LanguageEnglish
Pages784-788
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume259
DOIs
Publication statusPublished - 2018 Apr 15

Fingerprint

resonance fluorescence
bioinstrumentation
Silver
Biosensors
energy transfer
silver
Ions
proteins
Proteins
ions
Dimerization
Water
taps
Ponds
dimerization
helices
Sepharose
water
Gels
selectivity

Keywords

  • CupR
  • Fluorescence resonance energy transfer
  • FRET
  • Silver biosensor

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions. / Chen, Yi Ju; Liu, Cheng You; Tsai, Dong Yu; Yeh, Yi Chun.

In: Sensors and Actuators, B: Chemical, Vol. 259, 15.04.2018, p. 784-788.

Research output: Contribution to journalArticle

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