A novel quantitative immunomagnetic reduction assay for Nervous necrosis virus

Shieh Yueh Yang, Jen Leih Wu, Chun Hsi Tso, Fang Huar Ngou, Hsin Yiu Chou, Fan Hua Nan, Herng Er Horng, Ming Wei Lu

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    Rapid, sensitive, and automatic detection platforms are among the major approaches of controlling viral diseases in aquaculture. An efficient detection platform permits the monitoring of pathogen spread and helps to enhance the economic benefits of commercial aquaculture. Nervous necrosis virus (NNV), the cause of viral encephalopathy and retinopathy, is among the most devastating aquaculture viruses that infect marine fish species worldwide. In the present study, a highly sensitive magnetoreduction assay was developed for detecting target biomolecules with a primary focus on NNV antigens. A standard curve of the different NNV concentrations that were isolated from infected Malabar grouper (Epinephelus malabaricus) was established before experiments were conducted. The test solution was prepared by homogeneous dispersion of magnetic nanoparticles coated with rabbit anti-NNV antibody. The magnetic nanoparticles in the solution were oscillated by magnetic interaction with multiple externally applied, alternating current magnetic fields. The assay's limit of detection was approximately 2 × 101 TCID50/ml for NNV. Moreover, the immunomagnetic reduction readings for other aquatic viruses (i.e., 1 × 107 TCID50/ml for Infectious pancreatic necrosis virus and 1 × 106.5 TCID50/ml for grouper iridovirus) were below the background noise in the NNV solution, demonstrating the specificity of the new detection platform.

    Original languageEnglish
    Pages (from-to)911-917
    Number of pages7
    JournalJournal of Veterinary Diagnostic Investigation
    Volume24
    Issue number5
    DOIs
    Publication statusPublished - 2012 Sep

    Keywords

    • Immunomagnetic reduction
    • magnetic beads

    ASJC Scopus subject areas

    • veterinary(all)

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