A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells

Shen Jeu Won, Ting Yu Lin, Cheng Hsin Yen, Yu Hau Tzeng, Hsiao Sheng Liu, Chun Nan Lin, Cheng Hao Yu, Chi Shiuan Wu, Jyun Ti Chen, Yi Ting Chen, Chi Ying F. Huang, Chun Li Su

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5 Citations (Scopus)

Abstract

The tautomeric pair of garcinielliptone FC (GFC) is a pure and structure-defined active ingredient, isolated from Garcinia subelliptica Merr., a plant with abundant sources of polyphenols. Here, we demonstrate that a higher concentration of GFC had no effect on normal human umbilical vein endothelial cells. On chemoresistant human colorectal cancer HT-29 cells, a time- and dosage-related cytotoxicity was observed. GFC also inhibited the nuclear expression and DNA binding activity of nuclear factor κB in a time- and dosage-related manner using Western blot and electrophoretic mobility shift assay. Furthermore, GFC induced apoptosis, which was characterized by an increase of cells at the sub-G1 phase, induction of DNA fragmentation, and formation of apoptotic bodies using flow cytometry, electrophoresis, and fluorescence microscopy, respectively. Western blot analysis revealed that both caspase-dependent intrinsic and extrinsic pathways as well as caspase-independent pathway were involved. Our data suggest the anticancer mechanisms of GFC on human colorectal cancer.

Original languageEnglish
Pages (from-to)568-578
Number of pages11
JournalJournal of Functional Foods
Volume24
DOIs
Publication statusPublished - 2016 Jun 1

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Keywords

  • Apoptosis
  • Garcinielliptone FC
  • Human colorectal cancer
  • Nuclear factor κB

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Won, S. J., Lin, T. Y., Yen, C. H., Tzeng, Y. H., Liu, H. S., Lin, C. N., Yu, C. H., Wu, C. S., Chen, J. T., Chen, Y. T., Huang, C. Y. F., & Su, C. L. (2016). A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells. Journal of Functional Foods, 24, 568-578. https://doi.org/10.1016/j.jff.2016.05.003