This study proposed a novel approach to replace the traditional surface plasmon resonance (SPR) bulk prism by microlens arrays (MLAs). It demonstrated the effect that coupling SPR on the optical response of microlens arrays structure. Surface plasmons are features specific to the interface of metal-dielectric. They are due to charge density oscillations in the metal, accompanied by electromagnetic field dissipation in the metal and in the dielectric. SPR biosensor bulk prism technology has been commercialized and SPR biosensors have become a central tool for characterizing and quantifying biomolecular interactions. We will used this microlens arrays coupling SPP phenomenon, which gives rise to selective spectral response due to a local field enhancement interrelating the optical and biochemical domains.