A novel method for detection of fish iridovirus in groupers using nano-magnetic particles

Ming Wei Lu; Hsiang Ping Kuo; Hsin Yiu Chou; Ee Ying Ling; Chia Ling Chung; Yu Fang Hung; Herng Er Horng; Jen Leih Wu; Guor Mour Her; Zwe Ling Kong

doi:10.3147/jsfp.51.S54

A novel method for detection of fish iridovirus in groupers using nano-magnetic particles

Ming Wei Lu^*, Hsiang Ping Kuo, Hsin Yiu Chou, Ee Ying Ling, Chia Ling Chung, Yu Fang Hung, Herng Er Horng, Jen Leih Wu, Guor Mour Her, Zwe Ling Kong

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 101 TCID50/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 19% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.

Original language	English
Pages (from-to)	S54-S59
Journal	Fish Pathology
Volume	51
DOIs	https://doi.org/10.3147/jsfp.51.S54
Publication status	Published - 2016

Keywords

Immunomagnetic reduction
Iridovirus
Magnetic nanoparticles
Real-time polymerase chain reaction

ASJC Scopus subject areas

Aquatic Science
Animal Science and Zoology

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10.3147/jsfp.51.S54

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title = "A novel method for detection of fish iridovirus in groupers using nano-magnetic particles",

abstract = "Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 101 TCID50/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 19% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.",

keywords = "Immunomagnetic reduction, Iridovirus, Magnetic nanoparticles, Real-time polymerase chain reaction",

author = "Lu, {Ming Wei} and Kuo, {Hsiang Ping} and Chou, {Hsin Yiu} and Ling, {Ee Ying} and Chung, {Chia Ling} and Hung, {Yu Fang} and Horng, {Herng Er} and Wu, {Jen Leih} and Her, {Guor Mour} and Kong, {Zwe Ling}",

note = "Publisher Copyright: {\textcopyright} 2016 The Japanese Society of Fish Pathology.",

year = "2016",

doi = "10.3147/jsfp.51.S54",

language = "English",

volume = "51",

pages = "S54--S59",

journal = "Fish Pathology",

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publisher = "Japanese Society of Fish Pathology",

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T1 - A novel method for detection of fish iridovirus in groupers using nano-magnetic particles

AU - Lu, Ming Wei

AU - Kuo, Hsiang Ping

AU - Chou, Hsin Yiu

AU - Ling, Ee Ying

AU - Chung, Chia Ling

AU - Hung, Yu Fang

AU - Horng, Herng Er

AU - Wu, Jen Leih

AU - Her, Guor Mour

AU - Kong, Zwe Ling

PY - 2016

Y1 - 2016

N2 - Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 101 TCID50/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 19% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.

AB - Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 101 TCID50/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 19% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.

KW - Immunomagnetic reduction

KW - Iridovirus

KW - Magnetic nanoparticles

KW - Real-time polymerase chain reaction

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SP - S54-S59

JO - Fish Pathology

JF - Fish Pathology

ER -

A novel method for detection of fish iridovirus in groupers using nano-magnetic particles

Abstract

Keywords

ASJC Scopus subject areas

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