A novel method for detection of fish iridovirus in groupers using nano-magnetic particles

Ming Wei Lu*, Hsiang Ping Kuo, Hsin Yiu Chou, Ee Ying Ling, Chia Ling Chung, Yu Fang Hung, Herng Er Horng, Jen Leih Wu, Guor Mour Her, Zwe Ling Kong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 101 TCID50/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 19% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.

Original languageEnglish
Pages (from-to)S54-S59
JournalFish Pathology
Publication statusPublished - 2016


  • Immunomagnetic reduction
  • Iridovirus
  • Magnetic nanoparticles
  • Real-time polymerase chain reaction

ASJC Scopus subject areas

  • Aquatic Science
  • Animal Science and Zoology


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