A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets

Maria A. Tikhonova*, Nikolai A. Maslov, Alim A. Bashirzade, Eugenyi V. Nehoroshev, Vladislav Y. Babchenko, Nadezhda D. Chizhova, Elena O. Tsibulskaya, Anna A. Akopyan, Evgeniya V. Markova, Yi Ling Yang, Kwok Tung Lu, Allan V. Kalueff, Lyubomir I. Aftanas, Tamara G. Amstislavskaya*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening.

Original languageEnglish
Article number1751
Issue number8
Publication statusPublished - 2022 Aug


  • animal model
  • behavior
  • laser
  • neurodegeneration
  • neuroinflammation
  • neurorepair
  • traumatic brain injury
  • zebrafish

ASJC Scopus subject areas

  • Pharmaceutical Science


Dive into the research topics of 'A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets'. Together they form a unique fingerprint.

Cite this