A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay

Bing Hsien Liu; Yu Chen Lin; Chia Shin Ho; Che Chuan Yang; Yun Tsui Chang; Jui Feng Chang; Chun Yuan Li; Cheng Shun Cheng; Jiun Yan Huang; Yen Fu Lee; Ming Hung Hsu; Feng Chun Lin; Hao Ching Wang; Chu Fang Lo; Shieh Yueh Yang; Han Ching Wang

doi:10.1371/journal.pone.0138207

A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay

Bing Hsien Liu, Yu Chen Lin, Chia Shin Ho, Che Chuan Yang, Yun Tsui Chang, Jui Feng Chang, Chun Yuan Li, Cheng Shun Cheng, Jiun Yan Huang, Yen Fu Lee, Ming Hung Hsu, Feng Chun Lin, Hao Ching Wang, Chu Fang Lo, Shieh Yueh Yang, Han Ching Wang

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 103 ng/ml, and the linear dynamic range of the assay was 0.1~1 x 106 ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.

Original language	English
Article number	e0138207
Journal	PloS one
Volume	10
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0138207
Publication status	Published - 2015 Sept 18

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences
General

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Liu, B. H., Lin, Y. C., Ho, C. S., Yang, C. C., Chang, Y. T., Chang, J. F., Li, C. Y., Cheng, C. S., Huang, J. Y., Lee, Y. F., Hsu, M. H., Lin, F. C., Wang, H. C., Lo, C. F., Yang, S. Y., & Wang, H. C. (2015). A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay. PloS one, 10(9), Article e0138207. https://doi.org/10.1371/journal.pone.0138207

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title = "A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay",

abstract = "Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 103 ng/ml, and the linear dynamic range of the assay was 0.1~1 x 106 ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.",

author = "Liu, {Bing Hsien} and Lin, {Yu Chen} and Ho, {Chia Shin} and Yang, {Che Chuan} and Chang, {Yun Tsui} and Chang, {Jui Feng} and Li, {Chun Yuan} and Cheng, {Cheng Shun} and Huang, {Jiun Yan} and Lee, {Yen Fu} and Hsu, {Ming Hung} and Lin, {Feng Chun} and Wang, {Hao Ching} and Lo, {Chu Fang} and Yang, {Shieh Yueh} and Wang, {Han Ching}",

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AU - Liu, Bing Hsien

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AU - Ho, Chia Shin

AU - Yang, Che Chuan

AU - Chang, Yun Tsui

AU - Chang, Jui Feng

AU - Li, Chun Yuan

AU - Cheng, Cheng Shun

AU - Huang, Jiun Yan

AU - Lee, Yen Fu

AU - Hsu, Ming Hung

AU - Lin, Feng Chun

AU - Wang, Hao Ching

AU - Lo, Chu Fang

AU - Yang, Shieh Yueh

AU - Wang, Han Ching

PY - 2015/9/18

Y1 - 2015/9/18

N2 - Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 103 ng/ml, and the linear dynamic range of the assay was 0.1~1 x 106 ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.

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A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay

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