A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

C. H. Tsai Chih-Hsin; Po Han Cha; Cheng Huang Lin; Ta Chau Chang; Chih Ta Chia

doi:10.1002/elps.200600393

A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

C. H. Tsai Chih-Hsin, Po Han Cha, Cheng Huang Lin^*, Ta Chau Chang, Chih Ta Chia

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm^-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.

Original language	English
Pages (from-to)	4688-4693
Number of pages	6
Journal	Electrophoresis
Volume	27
Issue number	23
DOIs	https://doi.org/10.1002/elps.200600393
Publication status	Published - 2006 Dec

Keywords

CE
Malachite green
Raman spectroscopy
Surface enhanced-resonance Roman spectroscopy
Sweeping-MEKC

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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title = "A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode",

abstract = "A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.",

keywords = "CE, Malachite green, Raman spectroscopy, Surface enhanced-resonance Roman spectroscopy, Sweeping-MEKC",

author = "{Tsai Chih-Hsin}, {C. H.} and Cha, {Po Han} and Lin, {Cheng Huang} and Chang, {Ta Chau} and Chia, {Chih Ta}",

year = "2006",

month = dec,

doi = "10.1002/elps.200600393",

language = "English",

volume = "27",

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journal = "Electrophoresis",

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T1 - A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

AU - Tsai Chih-Hsin, C. H.

AU - Cha, Po Han

AU - Lin, Cheng Huang

AU - Chang, Ta Chau

AU - Chia, Chih Ta

PY - 2006/12

Y1 - 2006/12

N2 - A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.

AB - A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.

KW - CE

KW - Malachite green

KW - Raman spectroscopy

KW - Surface enhanced-resonance Roman spectroscopy

KW - Sweeping-MEKC

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A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

Abstract

Keywords

ASJC Scopus subject areas

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