A new approach for the detection of a nonfluorescent compound by CE-resonance Raman spectroscopy based on the sweeping-MEKC mode

C. H. Tsai Chih-Hsin, Po Han Cha, Cheng Huang Lin, Ta Chau Chang, Chih Ta Chia

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A CE-resonance Raman spectroscopy (CE-RRS) method based on MEKC and sweeping-MEKC modes is described. A nonfluorescent compound, malachite green (MG), and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as model compound and light source, respectively. In order to carry out a quantitative analysis of MG, a monochromator (effective bandwidth, 0.4 nm) was used to collect the specific Raman line at 1616 cm-1 (N-(φ and C-C stretch, corresponding to 582 nm when the wavelength of the exciting source was 532 nm). As a result, the LOD for MG was 10 ppm, based on the MEKC/RRS mode. This could be improved to 5 ppb when the sweeping-MEKC/RRS mode was applied. Furthermore, with the addition of nano-size silver colloids to the CE buffer the detection limits can be further improved, but the data obtained with surface-enhanced\resonance Raman spectroscopy (SERRS) are less useful for quantitative purposes.

Original languageEnglish
Pages (from-to)4688-4693
Number of pages6
JournalElectrophoresis
Volume27
Issue number23
DOIs
Publication statusPublished - 2006 Dec 1

Keywords

  • CE
  • Malachite green
  • Raman spectroscopy
  • Surface enhanced-resonance Roman spectroscopy
  • Sweeping-MEKC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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